Anti-KRT25 antibody - C-terminal (ab170330)

Overview

  • Product name
    Anti-KRT25 antibody - C-terminal
    See all KRT25 primary antibodies
  • Description
    Rabbit polyclonal to KRT25 - C-terminal
  • Tested applications
    Suitable for: Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human KRT25 aa 387-426 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q7Z3Z0

  • Positive control
    • K562 cell line lysate; K562 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    ab170330 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/100 - 1/500. Predicted molecular weight: 49 kDa.

Target

  • Relevance
    Essential for the proper assembly of type I and type II keratin protein complexes and formation of keratin intermediate filaments in the inner root sheath (irs).
  • Cellular localization
    Cytoplasmic
  • Database links
  • Alternative names
    • CK 25 antibody
    • Cytokeratin 25 antibody
    • K25 antibody
    • K25A antibody
    • Keratin 25 antibody
    • Keratin 25A antibody
    • Keratin, type I cytoskeletal 25 antibody
    • KRT25A antibody
    • type I inner root sheath specific keratin 25 irs1 antibody
    • Type I inner root sheath specific keratin K25irs1 antibody
    see all

Images

  • Flow cytometric analysis of K562 cells (right histogram) labeling KRT25 with ab170330 at 1/10 dilution compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Anti-KRT25 antibody - C-terminal (ab170330) at 1/100 dilution + K562 cell line lysates at 35 µg

    Predicted band size : 49 kDa

References

ab170330 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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