Anti-K33A antibody (ab233139)
Key features and details
- Rabbit polyclonal to K33A
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-K33A antibody -
Description
Rabbit polyclonal to K33A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chimpanzee -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human K33A aa 50-400. Expressed in E.coli.
Database link: O76009 -
Positive control
- WB: Recombinant human K33A protein; Human lung lysate; Mouse testis lysate. IHC-P: Human prostate cancer, kidney, cerebrum, glioma, liver and liver cancer tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233139 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 46 kDa.
|
|
IHC-P |
Use a concentration of 5 - 20 µg/ml.
|
Notes |
---|
WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 46 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
-
Tissue specificity
Expressed in the hair follicles. -
Sequence similarities
Belongs to the intermediate filament family. - Information by UniProt
-
Database links
- Entrez Gene: 743775 Chimpanzee
- Entrez Gene: 3883 Human
- Entrez Gene: 71888 Mouse
- Omim: 602761 Human
- SwissProt: A5A6P3 Chimpanzee
- SwissProt: O76009 Human
- SwissProt: Q8K0Y2 Mouse
- Unigene: 512579 Human
see all -
Alternative names
- Ha-3I antibody
- HA3I antibody
- Hair keratin antibody
see all
Images
-
Anti-K33A antibody (ab233139) at 2 µg + Recombinant human K33A protein
Predicted band size: 46 kDa -
Anti-K33A antibody (ab233139) at 1 µg/ml + Human lung lysate
Predicted band size: 46 kDa -
Anti-K33A antibody (ab233139) at 1 µg + Mouse testis lysate
Predicted band size: 46 kDa -
Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for K33A using ab233139 at 20 μg/ml in immunohistochemical analysis. DAB staining.
-
Formalin-fixed, paraffin-embedded human kidney tissue stained for K33A using ab233139 at 20 μg/ml in immunohistochemical analysis. DAB staining.
-
Formalin-fixed, paraffin-embedded human cerebrum tissue stained for K33A using ab233139 at 20 μg/ml in immunohistochemical analysis. DAB staining.
-
Formalin-fixed, paraffin-embedded human glioma tissue stained for K33A using ab233139 at 20 μg/ml in immunohistochemical analysis. DAB staining.
-
Formalin-fixed, paraffin-embedded human liver tissue stained for K33A using ab233139 at 20 μg/ml in immunohistochemical analysis. DAB staining.
-
Formalin-fixed, paraffin-embedded human liver cancer tissue stained for K33A using ab233139 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab233139 has not yet been referenced specifically in any publications.