Anti-Krt73 antibody - C-terminal (ab170331)

Overview

  • Product name
    Anti-Krt73 antibody - C-terminal
    See all Krt73 primary antibodies
  • Description
    Rabbit polyclonal to Krt73 - C-terminal
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Krt73 aa 500-530 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q86Y46

  • Positive control
    • K562 cell line lysates and mouse heart tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab170331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 58 kDa.

Target

  • Function
    Has a role in hair formation. Specific component of keratin intermediate filaments in the inner root sheath (IRS) of the hair follicle.
  • Tissue specificity
    Highly expressed in hair follicles from scalp. In hair, it is specifically present in the inner root sheath (IRS) of the hair follicle. Present in the IRS cuticle, but not in Henle or Huxley layers of the IRS. In the IRS cuticle, it is expressed between the lowermost bulb region of the cuticle and the region where Henle cells undergo abrupt terminal differentiation. Detected up to the uppermost cortex region where cuticle cells terminally differentiate (at protein level).
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Information by UniProt
  • Database links
  • Alternative names
    • CK-73 antibody
    • Cytokeratin-73 antibody
    • IRT6IRS3 antibody
    • K2C73_HUMAN antibody
    • K6IRS3 antibody
    • K73 antibody
    • Keratin 6 irs3 antibody
    • Keratin, type II cytoskeletal 73 antibody
    • Keratin-73 antibody
    • KRT6IRS3 antibody
    • Krt73 antibody
    • Type II inner root sheath-specific keratin-K6irs3 antibody
    • Type-II keratin Kb36 antibody
    see all

Images

  • Immunohistochemical analysis of formalin fixed and paraffin embedded mouse heart tissue labeling Krt73 with ab170331 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Anti-Krt73 antibody - C-terminal (ab170331) at 1/100 dilution + K562 cell line lysate at 35 µg

    Predicted band size : 58 kDa

References

ab170331 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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