Overview

  • Product name
  • Description
    Rabbit polyclonal to KRTAP25-1
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 1-102 of Human KRTAP25-1.

  • Positive control
    • Human placenta tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab122172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    In the hair cortex, hair keratin intermediate filaments are embedded in an interfilamentous matrix, consisting of hair keratin-associated proteins (KRTAP), which are essential for the formation of a rigid and resistant hair shaft through their extensive disulfide bond cross-linking with abundant cysteine residues of hair keratins. The matrix proteins include the high-sulfur and high-glycine-tyrosine keratins.
  • Sequence similarities
    Belongs to the PMG family.
  • Information by UniProt
  • Database links
  • Alternative names
    • KAP25.1 antibody
    • Keratin-associated protein 25-1 antibody
    • KR251_HUMAN antibody
    • KRTAP25-1 antibody

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nuclear membrane, plasma membrane and cell junctions. Recommended concentration of ab122172 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122172 at 1/75 dilution staining KRTAP25-1 in Paraffin-embedded Human Placenta tissue by Immunohistochemistry.

References

ab122172 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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