Overview

  • Product name
  • Description
    Rabbit polyclonal to KRTHA3B
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 140-404 of Human KRTHA3B (Uniprot ID: Q14525)

  • Positive control
    • HeLa cells and whole cell lysate, paraffin-embedded Cal27 xenograft

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 79% PBS, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 46 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.
ICC/IF 1/100 - 1/1000.

Target

  • Sequence similarities
    Belongs to the intermediate filament family.
  • Information by UniProt
  • Database links
  • Alternative names
    • HA 3II antibody
    • HA3II antibody
    • Hair keratin antibody
    • Hair keratin type I Ha3 II antibody
    • Hard keratin type I 3II antibody
    • hHa3 II antibody
    • hHa3II antibody
    • HKA3B antibody
    • K33B antibody
    • Keratin 33B antibody
    • Keratin antibody
    • Keratin hair acidic 3B antibody
    • Keratin hair acidic3B antibody
    • Keratin type I cuticular Ha3 II antibody
    • Keratin-33B antibody
    • Keratin33B antibody
    • KRT 33B antibody
    • KRT33B antibody
    • KRTHA 3B antibody
    • KRTHA3A antibody
    • KRTHA3B antibody
    • KT33B_HUMAN antibody
    • type I cuticular Ha3-II antibody
    • type I Ha3-II antibody
    • Type I hair keratin 3B antibody
    see all

Images

  • Anti-KRTHA3B antibody (ab154339) at 1/10000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 46 kDa



    10% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed HeLa cells labeling KRTHA3B with ab154339 at 1/500 dilution (green) and counterstained with Hoechst 3342 in bottom panel (blue).
  • Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, labeling KRTHA3B with ab154339 at 1/500 dilution

References

ab154339 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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