Overview

  • Product name
    Anti-KSHV ORF45 antibody [2D4A5]
  • Description
    Mouse monoclonal [2D4A5] to KSHV ORF45
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Kaposi's sarcoma herpes virus
  • Immunogen

    Ni-NTA purified recombinant human KSHV ORF45 expressed in E. Coli strain BL21 (DE3).

  • Positive control
    • TPA induced BCBL-1 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Purified from tissue culture supernatant.
  • Clonality
    Monoclonal
  • Clone number
    2D4A5
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab36618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 78 kDa (predicted molecular weight: 50 kDa).
ELISA Use at an assay dependent dilution.

Target

  • Relevance
    Kaposi's sarcoma-associated herpes virus (KSHV) belongs to the gamma-herpesvirus subfamily of the Herpes viridae family and has been closely linked to the Kaposi's sarcoma, primary effusion lymphoma (PEL) and multicentric Castleman's disease. The genome of KSHV is 165-170 kb and contains at least 88 open reading frames. ORF45 protein encoded by an immediate-early gene in the KSHV genome is characterized as a phosphorylated protein, and it is localized in the cytoplasm of infected cells. Studies have shown that ORF45 Protein interacted with cellular IRF-7 and blocked virus-mediated phosphorylation and nuclear translocation of IRF-7. In consequence, ORF45 efficiently inhibited virus-induced production of type I IFN. Zhu et al (2003) reported that the ORF45 Protein was associated with purified virions.
  • Cellular localization
    Not applicable
  • Alternative names
    • HHV8 antibody
    • Human herpes virus 8 antibody
    • Kaposi's sarcoma herpes virus antibody
    • KSHV antibody
    • ORF45 antibody
    see all

Images

  • All lanes : Anti-KSHV ORF45 antibody [2D4A5] (ab36618)

    Lane 1 : Molecular weight marker
    Lane 2 : Recombinant KSHV ORF45 expressed in E.coli


    Predicted band size : 50 kDa
    Observed band size : 78 kDa (why is the actual band size different from the predicted?)
    Based on the analysis of the mRNA sequence, ORF45 is expected to encode a protein of 407 amino acids with a molecular mass of about 50 kDa. However, ORF45 was displayed as a diffuse band at the position of 78 kDa on a Western blot after SDS-PAGE. This observation implied that ORF45 might be highly post-translationally modified, resulting in its reduced mobility in denaturing SDS-PAGE. A survey of the primary amino acid sequence of ORF45 revealed multiple potential sites of phosphorylation, including a serine-rich region and other consensus sites for phosphorylation by the serine/threonine kinase, casein kinase II, and protein kinase C (Zhu & Yuan, 2003).
  • Immunochemistry staining of TPA-induced BCBL-1 cells, using ab36618. HRP-anti-mouse was used as the secondary antibody before colour development with DAB.

References

This product has been referenced in:
  • Botto S  et al. Kaposi Sarcoma Herpesvirus Induces HO-1 during De Novo Infection of Endothelial Cells via Viral miRNA-Dependent and -Independent Mechanisms. MBio 6:e00668 (2015). Read more (PubMed: 26045540) »
  • Lin X  et al. MicroRNAs and unusual small RNAs discovered in Kaposi's sarcoma-associated herpesvirus virions. J Virol 86:12717-30 (2012). Read more (PubMed: 22973026) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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