The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 78 kDa (predicted molecular weight: 50 kDa).
Use at an assay dependent dilution.
RelevanceKaposi's sarcoma-associated herpes virus (KSHV) belongs to the gamma-herpesvirus subfamily of the Herpes viridae family and has been closely linked to the Kaposi's sarcoma, primary effusion lymphoma (PEL) and multicentric Castleman's disease. The genome of KSHV is 165-170 kb and contains at least 88 open reading frames. ORF45 protein encoded by an immediate-early gene in the KSHV genome is characterized as a phosphorylated protein, and it is localized in the cytoplasm of infected cells. Studies have shown that ORF45 Protein interacted with cellular IRF-7 and blocked virus-mediated phosphorylation and nuclear translocation of IRF-7. In consequence, ORF45 efficiently inhibited virus-induced production of type I IFN. Zhu et al (2003) reported that the ORF45 Protein was associated with purified virions.
Cellular localizationNot applicable
Human herpes virus 8 antibody
Kaposi's sarcoma herpes virus antibody
Anti-KSHV ORF45 antibody [2D4A5] images
Western blot - KSHV ORF45 antibody [2D4A5] (ab36618)
All lanes : Anti-KSHV ORF45 antibody [2D4A5] (ab36618)
Lane 1 : Molecular weight marker Lane 2 : Recombinant KSHV ORF45 expressed in E.coli
Immunochemistry staining of TPA-induced BCBL-1 cells, using ab36618. HRP-anti-mouse was used as the secondary antibody before colour development with DAB.
References for Anti-KSHV ORF45 antibody [2D4A5] (ab36618)
This product has been referenced in:
Abada R et al. SIAH-1 interacts with the Kaposi's sarcoma-associated herpesvirus-encoded ORF45 protein and promotes its ubiquitylation and proteasomal degradation. J Virol82:2230-40 (2008).
Read more (PubMed: 18077711) »