Overview

  • Product nameAnti-KSR1 antibody
    See all KSR1 primary antibodies
  • Description
    Rabbit polyclonal to KSR1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Dog, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Human KSR1.

  • Positive control
    • This antibody gave a positive signal in Mouse brain tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab98204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 97 kDa).

Target

  • FunctionLocation-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity.
  • Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated on Ser-309 and, to a higher extent, on Ser-404 by MARK3. Dephosphorylated on Ser-404 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated.
  • Cellular localizationCytoplasm. Membrane. In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery.
  • Information by UniProt
  • Database links
  • Alternative names
    • BKSR 1 antibody
    • BKSR1 antibody
    • dKsr antibody
    • EK 31 antibody
    • EK31 antibody
    • hb antibody
    • Kinase suppressor of Ras 1 antibody
    • Kinase suppressor of ras antibody
    • KSR 1 antibody
    • KSR antibody
    • KSR1 antibody
    • KSR1_HUMAN antibody
    • Positive Ras signaling mediator family member (86.4 kD) antibody
    • Positive Ras signaling mediator family member antibody
    • RSU 2 antibody
    • RSU2 antibody
    • SR 31 antibody
    • SR31 antibody
    • Suppressor of activated let 60 Ras SUR3 antibody
    • Suppressor of Ras1 31 antibody
    see all

Anti-KSR1 antibody images

  • Anti-KSR1 antibody (ab98204) at 1 µg/ml + Brain (Mouse) Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 97 kDa
    Observed band size : 105 kDa (why is the actual band size different from the predicted?)


    Exposure time : 8 minutes

References for Anti-KSR1 antibody (ab98204)

ab98204 has not yet been referenced specifically in any publications.

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