Anti-KSR1 (phospho S392) antibody (ab52205)

Overview

  • Product nameAnti-KSR1 (phospho S392) antibody
    See all KSR1 primary antibodies
  • Description
    Rabbit polyclonal to KSR1 (phospho S392)
  • Specificityab52205 detects endogenous levels of KSR only when phosphorylated at serine 392.
  • Tested applicationsSuitable for: IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthesized phosphopeptide derived from human KSR around the phosphorylation site of serine 392 (T-E-SP-V-P).

  • Positive control
    • Human brain tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab52205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.

Target

  • FunctionLocation-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity.
  • Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated on Ser-309 and, to a higher extent, on Ser-404 by MARK3. Dephosphorylated on Ser-404 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated.
  • Cellular localizationCytoplasm. Membrane. In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery.
  • Information by UniProt
  • Database links
  • Alternative names
    • BKSR 1 antibody
    • BKSR1 antibody
    • dKsr antibody
    • EK 31 antibody
    • EK31 antibody
    • hb antibody
    • Kinase suppressor of Ras 1 antibody
    • Kinase suppressor of ras antibody
    • KSR 1 antibody
    • KSR antibody
    • KSR1 antibody
    • KSR1_HUMAN antibody
    • Positive Ras signaling mediator family member (86.4 kD) antibody
    • Positive Ras signaling mediator family member antibody
    • RSU 2 antibody
    • RSU2 antibody
    • SR 31 antibody
    • SR31 antibody
    • Suppressor of activated let 60 Ras SUR3 antibody
    • Suppressor of Ras1 31 antibody
    see all

Anti-KSR1 (phospho S392) antibody images

  • This image shows human brain tissue stained with ab52205 at a dilution of 1/50 - 1/100. Right hand image: tissue treated with immunising peptide; left hand image: untreated.

References for Anti-KSR1 (phospho S392) antibody (ab52205)

ab52205 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"