Anti-KSR1 (phospho S404) antibody [EP2358Y] (ab76248)


  • Product nameAnti-KSR1 (phospho S404) antibody [EP2358Y]
    See all KSR1 primary antibodies
  • Description
    Rabbit monoclonal [EP2358Y] to KSR1 (phospho S404)
  • Tested applicationsSuitable for: ICC/IF, WBmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic phospho-peptide corresponding to residues surrounding Serine 404 of human KSR1

  • Positive control
    • WB: HeLa cell lysates, untreated or treated with Calyculin A IF: Sh-SY-5Y cells
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


Associated products


Our Abpromise guarantee covers the use of ab76248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/10000. Predicted molecular weight: 102 kDa.
  • Application notesIs unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • FunctionLocation-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity.
    • Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 protein kinase domain.
    • Post-translational
      Phosphorylated on Ser-309 and, to a higher extent, on Ser-404 by MARK3. Dephosphorylated on Ser-404 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated.
    • Cellular localizationCytoplasm. Membrane. In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery.
    • Information by UniProt
    • Database links
    • Alternative names
      • BKSR 1 antibody
      • BKSR1 antibody
      • dKsr antibody
      • EK 31 antibody
      • EK31 antibody
      • hb antibody
      • Kinase suppressor of Ras 1 antibody
      • Kinase suppressor of ras antibody
      • KSR 1 antibody
      • KSR antibody
      • KSR1 antibody
      • KSR1_HUMAN antibody
      • Positive Ras signaling mediator family member (86.4 kD) antibody
      • Positive Ras signaling mediator family member antibody
      • RSU 2 antibody
      • RSU2 antibody
      • SR 31 antibody
      • SR31 antibody
      • Suppressor of activated let 60 Ras SUR3 antibody
      • Suppressor of Ras1 31 antibody
      see all

    Anti-KSR1 (phospho S404) antibody [EP2358Y] images

    • All lanes : Anti-KSR1 (phospho S404) antibody [EP2358Y] (ab76248) at 1/2000 dilution

      Lane 1 : HeLa cell lysate (untreated)
      Lane 2 : HeLa cell lysate, treated with Calyculin A

      Lysates/proteins at 10 µg per lane.

      goat anti-rabbit HRP labelled IgG at 1/1000 dilution

      Predicted band size : 102 kDa
      Observed band size : 110 kDa (why is the actual band size different from the predicted?)
    • Immunofluorescent staining of Sh-SY-5Y cells, using ab76248, at 1/100 dilution.

    References for Anti-KSR1 (phospho S404) antibody [EP2358Y] (ab76248)

    ab76248 has not yet been referenced specifically in any publications.

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