Anti-KSR1 (phospho S404) antibody [EP2358Y] (ab76248)

Overview

  • Product name
    Anti-KSR1 (phospho S404) antibody [EP2358Y]
    See all KSR1 primary antibodies
  • Description
    Rabbit monoclonal [EP2358Y] to KSR1 (phospho S404)
  • Tested applications
    Suitable for: ICC/IF, WBmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic phospho-peptide corresponding to residues surrounding Serine 404 of human KSR1

  • Positive control
    • WB: HeLa cell lysates, untreated or treated with Calyculin A IF: Sh-SY-5Y cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab76248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/10000. Predicted molecular weight: 102 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function
      Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity.
    • Sequence similarities
      Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Phosphorylated on Ser-309 and, to a higher extent, on Ser-404 by MARK3. Dephosphorylated on Ser-404 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated.
    • Cellular localization
      Cytoplasm. Membrane. In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery.
    • Information by UniProt
    • Database links
    • Alternative names
      • BKSR 1 antibody
      • BKSR1 antibody
      • dKsr antibody
      • EK 31 antibody
      • EK31 antibody
      • hb antibody
      • Kinase suppressor of Ras 1 antibody
      • Kinase suppressor of ras antibody
      • KSR 1 antibody
      • KSR antibody
      • KSR1 antibody
      • KSR1_HUMAN antibody
      • Positive Ras signaling mediator family member (86.4 kD) antibody
      • Positive Ras signaling mediator family member antibody
      • RSU 2 antibody
      • RSU2 antibody
      • SR 31 antibody
      • SR31 antibody
      • Suppressor of activated let 60 Ras SUR3 antibody
      • Suppressor of Ras1 31 antibody
      see all

    Images

    • All lanes : Anti-KSR1 (phospho S404) antibody [EP2358Y] (ab76248) at 1/2000 dilution

      Lane 1 : HeLa cell lysate (untreated)
      Lane 2 : HeLa cell lysate, treated with Calyculin A

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP labelled IgG at 1/1000 dilution

      Predicted band size : 102 kDa
      Observed band size : 110 kDa (why is the actual band size different from the predicted?)
    • Immunofluorescent staining of Sh-SY-5Y cells, using ab76248, at 1/100 dilution.

    References

    ab76248 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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