Overview

  • Product name
  • Description
    Rabbit polyclonal to KSR2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human KSR2 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q6VAB6

  • Positive control
    • 293, K562, HepG2 or Hela lysate, Human brain tissue, MCF-7 cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Detects a band of approximately 108 kDa.

Target

  • Function
    Location-regulated scaffold connecting MEK to RAF. Blocks MAP3K8 kinase activity and MAP3K8-mediated signaling. Acts as a negative regulator of MAP3K3-mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production.
  • Tissue specificity
    Mainly expressed in brain and kidney.
  • Sequence similarities
    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
  • Domain
    The protein kinase domain is predicted to be catalytically inactive.
  • Post-translational
    modifications
    Phosphorylated on Ser-474 by MARK3.
  • Cellular localization
    Cytoplasm. Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ25965 antibody
    • hKSR 2 antibody
    • hKSR2 antibody
    • Kinase suppressor of Ras 2 antibody
    • KSR 2 antibody
    • Ksr2 antibody
    • KSR2_HUMAN antibody
    see all

Images

  • Flow cytometry analysis of MCF-7 cells (green) compared to a negative control cells (blue) using ab170442 at a 1/10 dilution. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Immunohistochemical analysis of paraffin embedded Human brain tissue labeling KSR2  with ab170442  at a 1/50 dilution.

  • All lanes : Anti-KSR2 antibody (ab170442) at 1/100 dilution

    Lane 1 : 293 cell line lysate
    Lane 2 : K562 cell line lysate
    Lane 3 : HepG2 cell line lysate
    Lane 4 : HeLa cell line lysate

    Lysates/proteins at 35 µg per lane.

References

ab170442 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab170442.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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