Overview

  • Product name
  • Description
    Rabbit polyclonal to KSR2
  • Specificity
    Detects endogenous levels of total KSR2 protein.
  • Tested applications
    Suitable for: WB, ELISA, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide from an internal region of human KSR2

  • Positive control
    • WB: 293 cell extract ICC/IF: HeLa cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab72753 was Affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa).
ELISA 1/40000.
ICC/IF 1/500 - 1/1000.
IP Use a concentration of 5 µg/ml.

Target

  • Function
    Location-regulated scaffold connecting MEK to RAF. Blocks MAP3K8 kinase activity and MAP3K8-mediated signaling. Acts as a negative regulator of MAP3K3-mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production.
  • Tissue specificity
    Mainly expressed in brain and kidney.
  • Sequence similarities
    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
  • Domain
    The protein kinase domain is predicted to be catalytically inactive.
  • Post-translational
    modifications
    Phosphorylated on Ser-474 by MARK3.
  • Cellular localization
    Cytoplasm. Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ25965 antibody
    • hKSR 2 antibody
    • hKSR2 antibody
    • Kinase suppressor of Ras 2 antibody
    • KSR 2 antibody
    • Ksr2 antibody
    • KSR2_HUMAN antibody
    see all

Anti-KSR2 antibody images

  • All lanes : Anti-KSR2 antibody (ab72753) at 1/500 dilution

    Lane 1 : 293 cell extract
    Lane 2 : 293 cell extract with immunising peptide at 10 µg

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 108 kDa
    Observed band size : 108 kDa
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence analysis of KSR2 in HeLa cells, using ab72753 at 1/500 dilution. Right panel was also treated with immunising peptide.
  • KSR2 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 5µg of Rabbit polyclonal to KSR2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab72753.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 108kDa; KSR2

References for Anti-KSR2 antibody (ab72753)

This product has been referenced in:
  • Castellanos-Martín A  et al. Unraveling heterogeneous susceptibility and the evolution of breast cancer using a systems biology approach. Genome Biol 16:40 (2015). Read more (PubMed: 25853295) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab72753.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up