• Product nameAnti-Ku70 antibody
    See all Ku70 primary antibodies
  • Description
    Rabbit polyclonal to Ku70
  • Specificitydetects endogenous levels of total Ku70 protein.
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    synthesized non-phosphopeptide derived from human Ku70 around the phosphorylation site of serine 5 (W-E-SP-Y-Y).

  • Positive control
    • human breast carcinoma tissue, extracts from HeLa cells


Associated products


Our Abpromise guarantee covers the use of ab63540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
WB 1/500 - 1/1000. Predicted molecular weight: 70 kDa.
IHC-P 1/50 - 1/100.


  • FunctionSingle stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
  • Sequence similaritiesBelongs to the ku70 family.
    Contains 1 Ku domain.
    Contains 1 SAP domain.
  • Developmental stageExpression does not increase during promyelocyte differentiation.
  • Post-translational
    Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair.
  • Cellular localizationNucleus. Chromosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5''-deoxyribose-5-phosphate lyase Ku70 antibody
    • 5''-dRP lyase Ku70 antibody
    • 70 kDa subunit of Ku antigen antibody
    • ATP dependent DNA helicase 2 subunit 1 antibody
    • ATP dependent DNA helicase II 70 kDa subunit antibody
    • ATP-dependent DNA helicase 2 subunit 1 antibody
    • ATP-dependent DNA helicase II 70 kDa subunit antibody
    • CTC box binding factor 75 kDa subunit antibody
    • CTC box-binding factor 75 kDa subunit antibody
    • CTC75 antibody
    • CTCBF antibody
    • DNA repair protein XRCC6 antibody
    • G22P1 antibody
    • Ku 70 antibody
    • Ku autoantigen p70 subunit antibody
    • Ku autoantigen, 70kDa antibody
    • Ku p70 antibody
    • Ku70 antibody
    • Ku70 DNA binding component of DNA-dependent proteinkinase complex (thyroid autoantigen 70 kDa antibody
    • Kup70 antibody
    • Lupus Ku autoantigen protein p70 antibody
    • ML8 antibody
    • Thyroid autoantigen 70kD (Ku antigen) antibody
    • Thyroid autoantigen antibody
    • Thyroid lupus autoantigen antibody
    • Thyroid lupus autoantigen p70 antibody
    • Thyroid-lupus autoantigen antibody
    • TLAA antibody
    • X ray repair complementing defective repair in Chinese hamster cells 6 antibody
    • X-ray repair complementing defective repair in Chinese hamster cells 6 antibody
    • X-ray repair cross-complementing protein 6 antibody
    • XRCC 6 antibody
    • Xrcc6 antibody
    • XRCC6_HUMAN antibody
    see all

Anti-Ku70 antibody images

  • All lanes : Anti-Ku70 antibody (ab63540) at 1/500 dilution

    Lane 1 : extracts from HeLa cells
    Lane 2 : extracts from HeLa cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 70 kDa
    Observed band size : 70 kDa
    Additional bands at : 28 kDa,65 kDa,80 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using 1/50 ab63540. Left: untreated sample. Right: sample treated with immunising peptide.

References for Anti-Ku70 antibody (ab63540)

ab63540 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab63540.
Please use the links above to contact us or submit feedback about this product.