The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIP: Use at a concentration of 2-5 µg/mg lysate.
WB: 1/2000 - 1/10000. Predicted molecular weight: 70 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionSingle stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
Sequence similaritiesBelongs to the ku70 family. Contains 1 Ku domain. Contains 1 SAP domain.
Developmental stageExpression does not increase during promyelocyte differentiation.
Post-translational modificationsPhosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair.
ATP dependent DNA helicase II 70 kDa subunit antibody
ATP-dependent DNA helicase 2 subunit 1 antibody
ATP-dependent DNA helicase II 70 kDa subunit antibody
CTC box binding factor 75 kDa subunit antibody
CTC box-binding factor 75 kDa subunit antibody
DNA repair protein XRCC6 antibody
Ku 70 antibody
Ku autoantigen p70 subunit antibody
Ku autoantigen, 70kDa antibody
Ku p70 antibody
Ku70 DNA binding component of DNA-dependent proteinkinase complex (thyroid autoantigen 70 kDa antibody
Lupus Ku autoantigen protein p70 antibody
Thyroid autoantigen 70kD (Ku antigen) antibody
Thyroid autoantigen antibody
Thyroid lupus autoantigen antibody
Thyroid lupus autoantigen p70 antibody
Thyroid-lupus autoantigen antibody
X ray repair complementing defective repair in Chinese hamster cells 6 antibody
X-ray repair complementing defective repair in Chinese hamster cells 6 antibody
X-ray repair cross-complementing protein 6 antibody
XRCC 6 antibody
Anti-Ku70 antibody images
Immunoprecipitation - Ku70 antibody (ab93809)
HeLa whole cell lysate (1mg) was immunoprecipitated using ab93809 at 3µg/mg. 20% of the immunoprecipitate was used for Western blotting. ab93809 was used at 0.4 µg/ml and the resulting band analysed by chemiluminescence with an exposure time of 1 second.
Western blot - Ku70 antibody (ab93809)
All lanes : Anti-Ku70 antibody (ab93809) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T cell lysate at 50 µg
Developed using the ECL technique
Predicted band size : 70 kDa
Exposure time : 3 seconds
References for Anti-Ku70 antibody (ab93809)
has not yet been referenced specifically in any publications.
Publishing research using ab93809? Please let us know so that we can cite the reference in this datasheet.
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