Anti-Ku70 antibody - Aminoterminal end (ab62820)

Overview

  • Product name
    Anti-Ku70 antibody - Aminoterminal end
    See all Ku70 primary antibodies
  • Description
    Goat polyclonal to Ku70 - Aminoterminal end
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    YKTEGDEEAEEEQ-C

    , corresponding to N terminal amino acids 8-20 of Human Ku70

  • Positive control
    • A431, HeLa and HepG2 cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab62820 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab62820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA: 1/2000.
    WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
    • Sequence similarities
      Belongs to the ku70 family.
      Contains 1 Ku domain.
      Contains 1 SAP domain.
    • Developmental stage
      Expression does not increase during promyelocyte differentiation.
    • Post-translational
      modifications
      Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair.
    • Cellular localization
      Nucleus. Chromosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • 5''-deoxyribose-5-phosphate lyase Ku70 antibody
      • 5''-dRP lyase Ku70 antibody
      • 70 kDa subunit of Ku antigen antibody
      • ATP dependent DNA helicase 2 subunit 1 antibody
      • ATP dependent DNA helicase II 70 kDa subunit antibody
      • ATP-dependent DNA helicase 2 subunit 1 antibody
      • ATP-dependent DNA helicase II 70 kDa subunit antibody
      • CTC box binding factor 75 kDa subunit antibody
      • CTC box-binding factor 75 kDa subunit antibody
      • CTC75 antibody
      • CTCBF antibody
      • DNA repair protein XRCC6 antibody
      • G22P1 antibody
      • Ku 70 antibody
      • Ku autoantigen p70 subunit antibody
      • Ku autoantigen, 70kDa antibody
      • Ku p70 antibody
      • Ku70 antibody
      • Ku70 DNA binding component of DNA-dependent proteinkinase complex (thyroid autoantigen 70 kDa antibody
      • Kup70 antibody
      • Lupus Ku autoantigen protein p70 antibody
      • ML8 antibody
      • Thyroid autoantigen 70kD (Ku antigen) antibody
      • Thyroid autoantigen antibody
      • Thyroid lupus autoantigen antibody
      • Thyroid lupus autoantigen p70 antibody
      • Thyroid-lupus autoantigen antibody
      • TLAA antibody
      • X ray repair complementing defective repair in Chinese hamster cells 6 antibody
      • X-ray repair complementing defective repair in Chinese hamster cells 6 antibody
      • X-ray repair cross-complementing protein 6 antibody
      • XRCC 6 antibody
      • Xrcc6 antibody
      • XRCC6_HUMAN antibody
      see all

    Images

    • Anti-Ku70 antibody - Aminoterminal end (ab62820) at 0.1 µg/ml + HeLa cell lysate (in RIPA buffer) at 35 µg

      Predicted band size : 70 kDa
      Observed band size : 70 kDa

    References

    ab62820 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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