Overview

  • Product nameAnti-Ku70 antibody [N3H10]
    See all Ku70 primary antibodies
  • Description
    Mouse monoclonal [N3H10] to Ku70
  • SpecificitySpecies reactivity note: Mouse & Hamster (Western blotting only).
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Hamster, Human, Xenopus laevis, Monkey
    Does not react with: Rabbit, Cow, Sea Urchin
  • Immunogen

    Human placental extract designated as PSE1-PL

  • EpitopeAmino acids 506-541.
  • Positive control
    • BT474, T47D, HepG2 or HeLa cells. Tonsil tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab80829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
IHC-P
ICC/IF
  • Application notesICC/IF: Use at an assay dependent dilution.
    IHC-P: 1/400 for 20-30 minutes at RT. Staining of formalin-fixed tissues is improved by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
    IP: Use at 2µg/mg of lysate (Native and denatured).
    WB: Use at a concentration of 0.25 - 0.5 µg/ml for 2hrs at RT. Predicted molecular weight: 70 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionSingle stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
    • Sequence similaritiesBelongs to the ku70 family.
      Contains 1 Ku domain.
      Contains 1 SAP domain.
    • Developmental stageExpression does not increase during promyelocyte differentiation.
    • Post-translational
      modifications
      Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair.
    • Cellular localizationNucleus. Chromosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • 5''-deoxyribose-5-phosphate lyase Ku70 antibody
      • 5''-dRP lyase Ku70 antibody
      • 70 kDa subunit of Ku antigen antibody
      • ATP dependent DNA helicase 2 subunit 1 antibody
      • ATP dependent DNA helicase II 70 kDa subunit antibody
      • ATP-dependent DNA helicase 2 subunit 1 antibody
      • ATP-dependent DNA helicase II 70 kDa subunit antibody
      • CTC box binding factor 75 kDa subunit antibody
      • CTC box-binding factor 75 kDa subunit antibody
      • CTC75 antibody
      • CTCBF antibody
      • DNA repair protein XRCC6 antibody
      • G22P1 antibody
      • Ku 70 antibody
      • Ku autoantigen p70 subunit antibody
      • Ku autoantigen, 70kDa antibody
      • Ku p70 antibody
      • Ku70 antibody
      • Ku70 DNA binding component of DNA-dependent proteinkinase complex (thyroid autoantigen 70 kDa antibody
      • Kup70 antibody
      • Lupus Ku autoantigen protein p70 antibody
      • ML8 antibody
      • Thyroid autoantigen 70kD (Ku antigen) antibody
      • Thyroid autoantigen antibody
      • Thyroid lupus autoantigen antibody
      • Thyroid lupus autoantigen p70 antibody
      • Thyroid-lupus autoantigen antibody
      • TLAA antibody
      • X ray repair complementing defective repair in Chinese hamster cells 6 antibody
      • X-ray repair complementing defective repair in Chinese hamster cells 6 antibody
      • X-ray repair cross-complementing protein 6 antibody
      • XRCC 6 antibody
      • Xrcc6 antibody
      • XRCC6_HUMAN antibody
      see all

    References for Anti-Ku70 antibody [N3H10] (ab80829)

    This product has been referenced in:
    • Ajmani AK  et al. Absence of autoantigen Ku in mature human neutrophils and human promyelocytic leukemia line (HL-60) cells and lymphocytes undergoing apoptosis. J Exp Med 181:2049-58 (1995). Read more (PubMed: 7759997) »
    • Wang J  et al. Murine monoclonal antibodies specific for conserved and non-conserved antigenic determinants of the human and murine Ku autoantigens. Mol Biol Rep 18:15-28 (1993). Read more (PubMed: 7694076) »

    See all 2 Publications for this product

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