Overview

  • Product nameAnti-Ku70 antibody [S5C11]
    See all Ku70 primary antibodies
  • Description
    Mouse monoclonal [S5C11] to Ku70
  • Tested applicationsSuitable for: ICC/IF, Flow Cyt, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Human, Xenopus laevis, Monkey
  • Immunogen

    Human B cell nuclei from plasmacytoid 2p68 cells.

  • Positive control
    • HeLa cells. Tonsil.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Constituents: 3% Sodium deoxycholate, 3% Triton-X-100, 0.3% Tris HCl, 15% Sodium chloride
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesPurified from ascites fluid by ammonium sulfate precipitation.
  • ClonalityMonoclonal
  • Clone numberS5C11
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
Flow Cyt Use 0.5µg for 106 cells.
IHC-P Use a concentration of 1 - 2 µg/ml. Antigen retrieval is not essential but may optimise staining.
IP Use at 2 µg/mg of lysate.

Target

  • FunctionSingle stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
  • Sequence similaritiesBelongs to the ku70 family.
    Contains 1 Ku domain.
    Contains 1 SAP domain.
  • Developmental stageExpression does not increase during promyelocyte differentiation.
  • Post-translational
    modifications
    Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair.
  • Cellular localizationNucleus. Chromosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5''-deoxyribose-5-phosphate lyase Ku70 antibody
    • 5''-dRP lyase Ku70 antibody
    • 70 kDa subunit of Ku antigen antibody
    • ATP dependent DNA helicase 2 subunit 1 antibody
    • ATP dependent DNA helicase II 70 kDa subunit antibody
    • ATP-dependent DNA helicase 2 subunit 1 antibody
    • ATP-dependent DNA helicase II 70 kDa subunit antibody
    • CTC box binding factor 75 kDa subunit antibody
    • CTC box-binding factor 75 kDa subunit antibody
    • CTC75 antibody
    • CTCBF antibody
    • DNA repair protein XRCC6 antibody
    • G22P1 antibody
    • Ku 70 antibody
    • Ku autoantigen p70 subunit antibody
    • Ku autoantigen, 70kDa antibody
    • Ku p70 antibody
    • Ku70 antibody
    • Ku70 DNA binding component of DNA-dependent proteinkinase complex (thyroid autoantigen 70 kDa antibody
    • Kup70 antibody
    • Lupus Ku autoantigen protein p70 antibody
    • ML8 antibody
    • Thyroid autoantigen 70kD (Ku antigen) antibody
    • Thyroid autoantigen antibody
    • Thyroid lupus autoantigen antibody
    • Thyroid lupus autoantigen p70 antibody
    • Thyroid-lupus autoantigen antibody
    • TLAA antibody
    • X ray repair complementing defective repair in Chinese hamster cells 6 antibody
    • X-ray repair complementing defective repair in Chinese hamster cells 6 antibody
    • X-ray repair cross-complementing protein 6 antibody
    • XRCC 6 antibody
    • Xrcc6 antibody
    • XRCC6_HUMAN antibody
    see all

Anti-Ku70 antibody [S5C11] images

  • ab3108 at a 1/200 dilution staining asynchronous HeLa by ICC/IF. The cells were bleomycin treated for 2 hours prior to formaldehyde fixation and then labelled with antibody for 30 minutes. Bound antibody was detected using a Cy3 conjugated goat anti-mouse antibody. Nuclei were visualised using DAPI staining. Ab3108 was found to stain both treated and untreated cells very well.

    This image is courtesy of an Abreview submitted by Kirk McManus.

    See Abreview

  • ab3108 used in Immunoprecipitation at a 1/20 dilution.
    Hela cytosolic and nuclear extracts were loaded at 500µg. Immunoprecipitation step performed using Protein G. A non-Abcam rabbit polyclonal anti-Ku70 conjugated to HRP was used at a 1/2000 and for Western Blot a mouse monoclonal anti-Ku80 was used at a 1/50 dilution.
  • IHC image of ab3108 staining in human breast formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3108, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Overlay histogram showing HeLa cells stained with ab3108 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3108, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-Ku70 antibody [S5C11] (ab3108)

This product has been referenced in:
  • Alshareeda AT  et al. Clinicopathological significance of KU70/KU80, a key DNA damage repair protein in breast cancer. Breast Cancer Res Treat 139:301-10 (2013). Human . Read more (PubMed: 23624778) »
  • Parvathaneni S  et al. Human RECQ1 interacts with Ku70/80 and modulates DNA end-joining of double-strand breaks. PLoS One 8:e62481 (2013). IP, ICC/IF ; Human . Read more (PubMed: 23650516) »

See all 7 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (intestine)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer, 120C for 10 min.
Permeabilization Yes - methanol
Specification intestine
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative 50% ethanol/5% acetic acid followed by formaldehyde fixation
Username

Amr Ghaleb

Verified customer

Submitted Jul 15 2015

Vielen Dank für Ihren Anruf gestern.

Ich habe mir das Datenblatt von ab3108 nochmals genau angeschaut und kann nun erklären warum beide Banden auf dem WB zu sehen sind:

Dieser WB ist nicht mit unserem ab3108 gemacht sond...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Hela cells)
Specification Hela cells
Fixative Methanol
Permeabilization No
Blocking step Fish scale gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 23 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (Hela cytosolic and nuclear extracts)
Total protein in input 500 µg
Specification Hela cytosolic and nuclear extracts
Immuno-precipitation step Protein G
Username

Abcam user community

Verified customer

Submitted Nov 03 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (testis)
Specification testis
Fixative formalin
Permeabilization Yes - PBS 1x, 1.5% BSA, 0.1% Triton
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted May 14 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

Submitted Jun 05 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"