Overview

  • Product nameAnti-Ku70 + Ku80 antibody [162]
    See all Ku70 + Ku80 primary antibodies
  • Description
    Mouse monoclonal [162] to Ku70 + Ku80
  • Tested applicationsSuitable for: Flow Cyt, ICC/IF, IHC-P, Othermore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Xenopus laevis, Monkey
    Does not react with: Rabbit, Cow
  • Immunogen

    Human B cell nuclei from plasmacytoid 2p68 cells.

  • EpitopeA conformational epitope of p70/p80 dimer, which is destroyed during Western blotting.
  • Positive control
    • Tonsil.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 10mM PBS, pH 7.4
  • Concentration information loading...
  • PurityAmmonium Sulphate Precipitation
  • Purification notesPurified from ascites fluid by ammonium sulfate precipitation.
  • ClonalityMonoclonal
  • Clone number162
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab80828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.
AP Use at an assay dependent concentration.
IHC-P Use a concentration of 1 - 2 µg/ml.
EMSA Use at an assay dependent concentration.
Other Use at an assay dependent concentration.
  • Application notesIs unsuitable for WB.
  • Target

    • RelevanceThe Ku autoantigen is a heterodimer of 70kDa (p70) and 80kDa (p80) proteins. The p70 / p80 dimer is important for function of a 460kDa DNA-dependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. The Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
    • Cellular localizationNuclear
    • Database links
    • Alternative names
      • CTC75 antibody
      • CTC85 antibody
      • CTCBF antibody
      • DNA repair protein XRCC5 antibody
      • DNA repair protein XRCC6 antibody
      • G22P1 antibody
      • G22P2 antibody
      • KARP1 antibody
      • Ku 70 antibody
      • Ku 80 antibody
      • Ku 86 antibody
      • Ku86 antibody
      • ML8 antibody
      • NFIV antibody
      • TLAA antibody
      • XRCC5 antibody
      • XRCC6 antibody
      see all

    Anti-Ku70 + Ku80 antibody [162] images

    • Formalin-fixed, paraffin-embedded human tonsil tissue stained with ab80828 at 1-2µg/ml using peroxidase-conjugate and AEC chromogen. Note nuclear staining of the cells.

    References for Anti-Ku70 + Ku80 antibody [162] (ab80828)

    This product has been referenced in:
    • Wang J  et al. Murine monoclonal antibodies specific for conserved and non-conserved antigenic determinants of the human and murine Ku autoantigens. Mol Biol Rep 18:15-28 (1993). ICC/IF, IHC-P, AP, Flow Cyt ; Human, Monkey, Mouse, Xenopus laevis, Rat . Read more (PubMed: 7694076) »
    • Reeves WH Use of monoclonal antibodies for the characterization of novel DNA-binding proteins recognized by human autoimmune sera. J Exp Med 161:18-39 (1985). ICC/IF, IHC-P, AP, Flow Cyt ; Human, Mouse, Rat, Monkey, Xenopus laevis . Read more (PubMed: 2578541) »

    See all 2 Publications for this product

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    There are currently no Abreviews or Questions for ab80828.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"