Overview

  • Product name
  • Description
    Rabbit polyclonal to Ku70 + Ku80
  • Specificity
    ab53126 detects endogenous levels of total Ku70 + Ku80 proteins.
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A synthetic peptide derived from human Ku70 + Ku80.

  • Positive control
    • LoVo cell extracts and human breast carcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab53126 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ELISA
  • Application notes
    ELISA: 1/20000.
    IHC-P: 1/50 - 1/100.
    WB: 1/300 - 1/1000. Detects bands of approximately 70 and 83 kDa (predicted molecular weight: 70 and 83 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      The Ku autoantigen is a heterodimer of 70kDa (p70) and 80kDa (p80) proteins. The p70 / p80 dimer is important for function of a 460kDa DNA-dependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. The Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
    • Cellular localization
      Nuclear
    • Database links
    • Alternative names
      • CTC75 antibody
      • CTC85 antibody
      • CTCBF antibody
      • DNA repair protein XRCC5 antibody
      • DNA repair protein XRCC6 antibody
      • G22P1 antibody
      • G22P2 antibody
      • KARP1 antibody
      • Ku 70 antibody
      • Ku 80 antibody
      • Ku 86 antibody
      • Ku86 antibody
      • ML8 antibody
      • NFIV antibody
      • TLAA antibody
      • XRCC5 antibody
      • XRCC6 antibody
      see all

    Images

    • All lanes : Anti-Ku70 + Ku80 antibody (ab53126) at 1/300 dilution

      Lane 1 : LoVo cell extract
      Lane 2 : LoVo cell extract with immunising peptide


      Predicted band size : 70 , 83 kDa
    • ab53126 at 1/50 dilution staining Ku70 + Ku80 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue in the absence and presence of the immunising peptide.

    References

    This product has been referenced in:
    • King HO  et al. RAD51 Is a Selective DNA Repair Target to Radiosensitize Glioma Stem Cells. Stem Cell Reports 8:125-139 (2017). WB . Read more (PubMed: 28076755) »
    • Marampon F  et al. Hypoxia sustains glioblastoma radioresistance through ERKs/DNA-PKcs/HIF-1a functional interplay. Int J Oncol 44:2121-31 (2014). Read more (PubMed: 24676782) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab53126.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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