Anti-Ku70 + Ku80 antibody - C-terminal (ab166822)

Overview

  • Product name
    Anti-Ku70 + Ku80 antibody - C-terminal
    See all Ku70 + Ku80 primary antibodies
  • Description
    Rabbit polyclonal to Ku70 + Ku80 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within the C terminal of Human Ku70 + Ku80 (P12956, P13010).

  • Positive control
    • LOVO cell extracts; Human kidney tissue; A549 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab166822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 70, 82 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/500.

Target

  • Relevance
    The Ku autoantigen is a heterodimer of 70kDa (p70) and 80kDa (p80) proteins. The p70 / p80 dimer is important for function of a 460kDa DNA-dependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. The Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • CTC75 antibody
    • CTC85 antibody
    • CTCBF antibody
    • DNA repair protein XRCC5 antibody
    • DNA repair protein XRCC6 antibody
    • G22P1 antibody
    • G22P2 antibody
    • KARP1 antibody
    • Ku 70 antibody
    • Ku 80 antibody
    • Ku 86 antibody
    • Ku86 antibody
    • ML8 antibody
    • NFIV antibody
    • TLAA antibody
    • XRCC5 antibody
    • XRCC6 antibody
    see all

Images

  • All lanes : Anti-Ku70 + Ku80 antibody - C-terminal (ab166822) at 1/500 dilution

    Lane 1 : LoVo cell lysate
    Lane 2 : LoVo cell lysate with synthesized peptide

    Predicted band size: 70, 82 kDa

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human kidney tissue labeling Ku70 + Ku80 with ab166822 at 5 µg/ml.
  • Immunofluorescence analysis of A549 cells labeling Ku70 + Ku80 with ab166822 at 1/100. The panel on the right is treated with the synthesized peptide.

References

ab166822 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab166822.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up