The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 83 kDa.
Use at 2 µg/mg of lysate.
Use at an assay dependent concentration. ab18434-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration.
FunctionSingle stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
Sequence similaritiesBelongs to the ku80 family. Contains 1 Ku domain.
Developmental stageExpression increases during promyelocyte differentiation.
DomainThe EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.
Post-translational modificationsPhosphorylated on serine residues. Phosphorylation by PRKDC may enhance helicase activity. Sumoylated.
ATP dependent DNA helicase II 80 kDa subunit antibody
ATP dependent DNA helicase II 86 Kd subunit antibody
ATP dependent DNA helicase II antibody
ATP-dependent DNA helicase 2 subunit 2 antibody
ATP-dependent DNA helicase II 80 kDa subunit antibody
CTC box binding factor 85 kDa antibody
CTC box-binding factor 85 kDa subunit antibody
DNA repair protein XRCC5 antibody
KARP 1 antibody
Ku 80 antibody
Ku autoantigen 80kDa antibody
Ku86 autoantigen related protein 1 antibody
KUB 2 antibody
Lupus Ku autoantigen protein p86 antibody
Nuclear factor IV antibody
Thyroid lupus autoantigen antibody
Thyroid-lupus autoantigen antibody
X ray repair complementing defective repair in Chinese hamster cells 5 (double strand break rejoining) antibody
X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) antibody
X-ray repair cross-complementing protein 5 antibody
Xray repair complementing defective repair in Chinese hamster cells 5 antibody
XRCC 5 antibody
References for Anti-Ku80 antibody  (Biotin) (ab79220)
This product has been referenced in:
Wang J et al. Murine monoclonal antibodies specific for conserved and non-conserved antigenic determinants of the human and murine Ku autoantigens. Mol Biol Rep18:15-28 (1993).
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