Overview

  • Product nameAnti-Ku80 antibody [5C5]
    See all Ku80 primary antibodies
  • Description
    Mouse monoclonal [5C5] to Ku80
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 61-340 of Human Ku80

  • Positive control
    • HeLa, MCF7, A549 and NIH3T3 cell lysates, Human tonsil and Human colon cancer tissues, Hela cells, recombinant Ku80 protein

Properties

Applications

Our Abpromise guarantee covers the use of ab119935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Detects a band of approximately 86 kDa (predicted molecular weight: 83 kDa).
ELISA 1/10000.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/200 - 1/1000.

Target

  • FunctionSingle stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
  • Sequence similaritiesBelongs to the ku80 family.
    Contains 1 Ku domain.
  • Developmental stageExpression increases during promyelocyte differentiation.
  • DomainThe EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.
  • Post-translational
    modifications
    Phosphorylated on serine residues. Phosphorylation by PRKDC may enhance helicase activity.
    Sumoylated.
  • Cellular localizationNucleus. Chromosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • 86 kDa subunit of Ku antigen antibody
    • ATP dependent DNA helicase 2 subunit 2 antibody
    • ATP dependent DNA helicase II 80 kDa subunit antibody
    • ATP dependent DNA helicase II 86 Kd subunit antibody
    • ATP dependent DNA helicase II antibody
    • ATP-dependent DNA helicase 2 subunit 2 antibody
    • ATP-dependent DNA helicase II 80 kDa subunit antibody
    • CTC box binding factor 85 kDa antibody
    • CTC box-binding factor 85 kDa subunit antibody
    • CTC85 antibody
    • CTCBF antibody
    • DNA repair protein XRCC5 antibody
    • KARP 1 antibody
    • KARP1 antibody
    • Ku 80 antibody
    • Ku autoantigen 80kDa antibody
    • Ku80 antibody
    • Ku86 antibody
    • Ku86 autoantigen related protein 1 antibody
    • KUB 2 antibody
    • KUB2 antibody
    • Lupus Ku autoantigen protein p86 antibody
    • NFIV antibody
    • Nuclear factor IV antibody
    • Thyroid lupus autoantigen antibody
    • Thyroid-lupus autoantigen antibody
    • TLAA antibody
    • X ray repair complementing defective repair in Chinese hamster cells 5 (double strand break rejoining) antibody
    • X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) antibody
    • X-ray repair cross-complementing protein 5 antibody
    • Xray repair complementing defective repair in Chinese hamster cells 5 antibody
    • XRCC 5 antibody
    • XRCC5 antibody
    • XRCC5_HUMAN antibody
    see all

Anti-Ku80 antibody [5C5] images

  • Immunohistochemical analysis of paraffin-embedded Human tonsil tissues using ab119935 at a dilution of 1/200 with DAB staining.
  • Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue using ab119935 at a dilution of 1/200 with DAB staining.
  • Immunofluorescence analysis of Hela cells using ab119935 at a dilution of 1/200 (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Flow cytometric analysis of Hela cells using ab119935 at a dilution of 1/200 (green) and negative control (purple).
  • ELISA, using ab119935 at a dilution of 1/10000.
  • Anti-Ku80 antibody [5C5] (ab119935) at 1/1000 dilution + Human vascular smooth muscle cell at 20 µg with Milk at 5 %

    Secondary
    Goat anti-mouse polyclonal IRDye® 800CW at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 83 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

    Image is courtesy of an anonymous AbReview.

    See Abreview

  • All lanes : Anti-Ku80 antibody [5C5] (ab119935) at 1/500 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : A549 cell lysate
    Lane 4 : NIH3T3 cell lysate


    Predicted band size : 83 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)

References for Anti-Ku80 antibody [5C5] (ab119935)

This product has been referenced in:
  • Beck C  et al. PARP3 affects the relative contribution of homologous recombination and nonhomologous end-joining pathways. Nucleic Acids Res 42:5616-32 (2014). WB ; Human . Read more (PubMed: 24598253) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (vascular smooth muscle cell)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification vascular smooth muscle cell
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Nov 04 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Na-citrate pH6
Sample Mouse Tissue sections (Liver, P5)
Specification Liver, P5
Permeabilization Yes - Triton 0,05%
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 29 2014

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris gel)
Sample Human Cell lysate - whole cell (MDA-MB-231 breast cancer cells)
Specification MDA-MB-231 breast cancer cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Aug 19 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"