Anti-Ku80 antibody [5C5] (ab119935)
Key features and details
- Mouse monoclonal [5C5] to Ku80
- Suitable for: WB, ELISA, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-Ku80 antibody [5C5]
See all Ku80 primary antibodies -
Description
Mouse monoclonal [5C5] to Ku80 -
Host species
Mouse -
Tested applications
Suitable for: WB, ELISA, IHC-P, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment, corresponding to amino acids 61-340 of Human Ku80
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Positive control
- HeLa, MCF7, A549 and NIH3T3 cell lysates, Human tonsil and Human colon cancer tissues, Hela cells, recombinant Ku80 protein
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General notes
This product was changed from ascites to supernatant. Lot no’s high than GR133722-19 are from Tissue Culture Supernatant
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
5C5 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab119935 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/500 - 1/2000. Detects a band of approximately 86 kDa (predicted molecular weight: 83 kDa).
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ELISA |
1/10000.
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IHC-P | (1) |
1/200 - 1/1000.
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Flow Cyt |
1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF | (3) |
1/200 - 1/1000.
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Notes |
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WB
1/500 - 1/2000. Detects a band of approximately 86 kDa (predicted molecular weight: 83 kDa). |
ELISA
1/10000. |
IHC-P
1/200 - 1/1000. |
Flow Cyt
1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/200 - 1/1000. |
Target
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Function
Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. -
Sequence similarities
Belongs to the ku80 family.
Contains 1 Ku domain. -
Developmental stage
Expression increases during promyelocyte differentiation. -
Domain
The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage. -
Post-translational
modificationsPhosphorylated on serine residues. Phosphorylation by PRKDC may enhance helicase activity.
Sumoylated. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 7520 Human
- Entrez Gene: 22596 Mouse
- Omim: 194364 Human
- SwissProt: P13010 Human
- SwissProt: P27641 Mouse
- Unigene: 388739 Human
- Unigene: 246952 Mouse
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Alternative names
- 86 kDa subunit of Ku antigen antibody
- ATP dependent DNA helicase 2 subunit 2 antibody
- ATP dependent DNA helicase II 80 kDa subunit antibody
see all
Images
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All lanes : Anti-Ku80 antibody [5C5] (ab119935) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : A549 cell lysate
Lane 4 : NIH3T3 cell lysate
Predicted band size: 83 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin-embedded Human tonsil tissues using ab119935 at a dilution of 1/200 with DAB staining.
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Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue using ab119935 at a dilution of 1/200 with DAB staining.
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Immunofluorescence analysis of Hela cells using ab119935 at a dilution of 1/200 (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Flow cytometric analysis of Hela cells using ab119935 at a dilution of 1/200 (green) and negative control (purple).
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ELISA, using ab119935 at a dilution of 1/10000.
Protocols
Datasheets and documents
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Datasheet download
References (11)
ab119935 has been referenced in 11 publications.
- Dhoonmoon A et al. The KU-PARP14 axis differentially regulates DNA resection at stalled replication forks by MRE11 and EXO1. Nat Commun 13:5063 (2022). PubMed: 36030235
- Guo Z et al. LncRNA linc00312 suppresses radiotherapy resistance by targeting DNA-PKcs and impairing DNA damage repair in nasopharyngeal carcinoma. Cell Death Dis 12:69 (2021). PubMed: 33431817
- Boivin M et al. Translation of GGC repeat expansions into a toxic polyglycine protein in NIID defines a novel class of human genetic disorders: The polyG diseases. Neuron 109:1825-1835.e5 (2021). PubMed: 33887199
- Dai PL et al. Different Proteins Regulated Apoptosis, Proliferation and Metastasis of Lung Adenocarcinoma After Radiotherapy at Different Time. Cancer Manag Res 12:2437-2447 (2020). PubMed: 32308480
- Xu S et al. CAS9 is a genome mutator by directly disrupting DNA-PK dependent DNA repair pathway. Protein Cell 11:352-365 (2020). PubMed: 32170574