Anti-Ku80 antibody [EPR3467] (ab79391)

Overview

  • Product nameAnti-Ku80 antibody [EPR3467]
    See all Ku80 primary antibodies
  • Description
    Rabbit monoclonal [EPR3467] to Ku80
  • SpecificityThe immunogen for ab79391 has 78.6% homology with mouse and rat ku80, therefore we do not guarantee reactivity in these species.
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in human Ku80

  • Positive control
    • A549 (treated with FBS), HeLa, U937 and HepG2 cell lysates; human breast carcinoma tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-Ku80 antibody (Alexa Fluor® 488) [EPR3467] (ab202659)

    Anti-Ku80 antibody (HRP) [EPR3467] (ab202359)

    Anti-Ku80 antibody (Alexa Fluor® 647) [EPR3467] (ab202660)

    Anti-Ku80 antibody (DyLight® 488)  [EPR3467] (ab139844)

Properties

Applications

Our Abpromise guarantee covers the use of ab79391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/500 - 1/2000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/150.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notesIs unsuitable for IP.
  • Target

    • FunctionSingle stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
    • Sequence similaritiesBelongs to the ku80 family.
      Contains 1 Ku domain.
    • Developmental stageExpression increases during promyelocyte differentiation.
    • DomainThe EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.
    • Post-translational
      modifications
      Phosphorylated on serine residues. Phosphorylation by PRKDC may enhance helicase activity.
      Sumoylated.
    • Cellular localizationNucleus. Chromosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • 86 kDa subunit of Ku antigen antibody
      • ATP dependent DNA helicase 2 subunit 2 antibody
      • ATP dependent DNA helicase II 80 kDa subunit antibody
      • ATP dependent DNA helicase II 86 Kd subunit antibody
      • ATP dependent DNA helicase II antibody
      • ATP-dependent DNA helicase 2 subunit 2 antibody
      • ATP-dependent DNA helicase II 80 kDa subunit antibody
      • CTC box binding factor 85 kDa antibody
      • CTC box-binding factor 85 kDa subunit antibody
      • CTC85 antibody
      • CTCBF antibody
      • DNA repair protein XRCC5 antibody
      • KARP 1 antibody
      • KARP1 antibody
      • Ku 80 antibody
      • Ku autoantigen 80kDa antibody
      • Ku80 antibody
      • Ku86 antibody
      • Ku86 autoantigen related protein 1 antibody
      • KUB 2 antibody
      • KUB2 antibody
      • Lupus Ku autoantigen protein p86 antibody
      • NFIV antibody
      • Nuclear factor IV antibody
      • Thyroid lupus autoantigen antibody
      • Thyroid-lupus autoantigen antibody
      • TLAA antibody
      • X ray repair complementing defective repair in Chinese hamster cells 5 (double strand break rejoining) antibody
      • X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) antibody
      • X-ray repair cross-complementing protein 5 antibody
      • Xray repair complementing defective repair in Chinese hamster cells 5 antibody
      • XRCC 5 antibody
      • XRCC5 antibody
      • XRCC5_HUMAN antibody
      see all

    Anti-Ku80 antibody [EPR3467] images

    • All lanes : Anti-Ku80 antibody [EPR3467] (ab79391) at 1/2000 dilution

      Lane 1 : A549 cell lysate, treated with FBS
      Lane 2 : HeLa cell lysate
      Lane 3 : U937 cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 83 kDa
      Observed band size : 83 kDa
    • ab79391 (1/200) staining Ku80 in Hela cells (green). Cells were fixed in methanol and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

      See Abreview

    • Overlay histogram showing HeLa cells stained with ab79391 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab79391, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • ab79391 at 1/250 dilution staining Ku80 in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.

    References for Anti-Ku80 antibody [EPR3467] (ab79391)

    ab79391 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Methanol
    Permeabilization No
    Username

    Dr. Kirk McManus

    Verified customer

    Submitted Feb 10 2012

    Thank you for contacting us.

    I can confirm that the abs have IgG1 isotype.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Thank you for contacting us and your interest in our products.

    As far as we know, rabbits do not have specific IgG subclasses like mice or humans. This rabbit monoclonal is therefore simply of the IgG class.

    I hope this informatio...

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Methanol
    Permeabilization No
    Username

    Dr. Kirk McManus

    Verified customer

    Submitted Nov 16 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"