• Product nameAnti-Ku80 antibody [EPR3468]
    See all Ku80 primary antibodies
  • Description
    Rabbit monoclonal [EPR3468] to Ku80
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • WB: lysates from A549, HeLa, HepG2 and MCF7 cells; IHC-P: Human tonsil tissue; ICC/IF: HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


    Alternative versions available:

    Anti-Ku80 antibody (Alexa Fluor® 488) [EPR3468] (ab198586)
    Anti-Ku80 antibody (Alexa Fluor® 647) [EPR3468] (ab198587)

    Anti-Ku80 antibody (HRP) [EPR3468] (ab199096)



Our Abpromise guarantee covers the use of ab80592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 83 kDa.
IP 1/50.
IHC-P 1/250 - 1/500.
ICC/IF 1/500 - 1/1000.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionSingle stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
    • Sequence similaritiesBelongs to the ku80 family.
      Contains 1 Ku domain.
    • Developmental stageExpression increases during promyelocyte differentiation.
    • DomainThe EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.
    • Post-translational
      Phosphorylated on serine residues. Phosphorylation by PRKDC may enhance helicase activity.
    • Cellular localizationNucleus. Chromosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • 86 kDa subunit of Ku antigen antibody
      • ATP dependent DNA helicase 2 subunit 2 antibody
      • ATP dependent DNA helicase II 80 kDa subunit antibody
      • ATP dependent DNA helicase II 86 Kd subunit antibody
      • ATP dependent DNA helicase II antibody
      • ATP-dependent DNA helicase 2 subunit 2 antibody
      • ATP-dependent DNA helicase II 80 kDa subunit antibody
      • CTC box binding factor 85 kDa antibody
      • CTC box-binding factor 85 kDa subunit antibody
      • CTC85 antibody
      • CTCBF antibody
      • DNA repair protein XRCC5 antibody
      • KARP 1 antibody
      • KARP1 antibody
      • Ku 80 antibody
      • Ku autoantigen 80kDa antibody
      • Ku80 antibody
      • Ku86 antibody
      • Ku86 autoantigen related protein 1 antibody
      • KUB 2 antibody
      • KUB2 antibody
      • Lupus Ku autoantigen protein p86 antibody
      • NFIV antibody
      • Nuclear factor IV antibody
      • Thyroid lupus autoantigen antibody
      • Thyroid-lupus autoantigen antibody
      • TLAA antibody
      • X ray repair complementing defective repair in Chinese hamster cells 5 (double strand break rejoining) antibody
      • X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) antibody
      • X-ray repair cross-complementing protein 5 antibody
      • Xray repair complementing defective repair in Chinese hamster cells 5 antibody
      • XRCC 5 antibody
      • XRCC5 antibody
      • XRCC5_HUMAN antibody
      see all

    Anti-Ku80 antibody [EPR3468] images

    • All lanes : Anti-Ku80 antibody [EPR3468] (ab80592) at 1/50000 dilution

      Lane 1 : A549 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 83 kDa
      Observed band size : 83 kDa
    • Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using 1/100 ab80592.
    • Immunofluorescence analysis of HeLa cells with 1/500 ab80592.

    References for Anti-Ku80 antibody [EPR3468] (ab80592)

    This product has been referenced in:
    • Ho SR  et al. RNF144A, an E3 ubiquitin ligase for DNA-PKcs, promotes apoptosis during DNA damage. Proc Natl Acad Sci U S A 111:E2646-55 (2014). Read more (PubMed: 24979766) »
    • Grundy GJ  et al. APLF promotes the assembly and activity of non-homologous end joining protein complexes. EMBO J 32:112-25 (2013). Read more (PubMed: 23178593) »

    See all 3 Publications for this product

    Product Wall

    Thank you for contacting us with your enquiry. I have attached the CoC for ab80592 lot *** to this email. Please let me know if you have any questions or if there is anything else that we can do for you.