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Products:Neuroscience >> Neurotransmission >> Receptors / Channels >> Potassium Channels
Overview
Product name
Kv1.6 peptide
Protein description
Synthetic peptide derived from within residues 500 to the C-terminus of Human Kv1.6. (Note: the amino acid sequence is proprietary)
Protein length
529 amino acids
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Information available upon request.
Concentration
Concentration information loading...
Research areas
Neuroscience >> Neurotransmission >> Receptors / Channels >> Potassium Channels
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Western blot
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab23431 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking
Application notes
BL: Use at a concentration of 1 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
Function
Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.
Sequence similarities
Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.6/KCNA6 sub-subfamily.
Domain
The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
Cellular localization
Membrane.
Target information above from: UniProt accessionP17658
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Kv1.6 peptide images:
Western blot
All lanes : Anti-Kv1.6 antibody (ab21089) at 1 µg/ml
Lane 1 : Mouse Brain Lysate
Lane 2 : Mouse Brain Lysate with Kv1.6 peptide (ab23431) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
Lane 1 : Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
Lane 2 : Alexa Fluor Goat polyclonal to Rabbit IgG
Predicted band size : 58 kDa
Observed band size : 58 kDa
Additional bands at : 35 kDa (possible cleavage fragment,non-specific binding,degradation product),39 kDa (possible cleavage fragment,non-specific binding,degradation product),45 kDa (possible cleavage fragment,non-specific binding,degradation product).
ab21089 recognises a band corresponding to the predicted MW of Kv1.6 in mouse brain lysate (lane 1). Antibody binding to this band may be blocked using the immunising peptide, ab23431 (lane 2). The antibody also recognises additional proteins. These may be degradation/cleavage products of Kv1.6, or they may represent non-specific binding by the antibody.
References for Kv1.6 peptide (ab23431)
ab23431 has not yet been referenced specifically in any publications.
Publishing research using ab23431? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"