Anti-Kv1.4 antibody (ab16718)

Overview

  • Product nameAnti-Kv1.4 antibody
    See all Kv1.4 primary antibodies
  • Description
    Rabbit polyclonal to Kv1.4
  • Tested applicationsSuitable for: ELISA, IP, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant fragment:

    CNSHMPYGYAAQARARERERLAHSR

    , corresponding to amino acids 13-37 of Rat Kv1.4 (conjugated to KLH).

  • Positive control
    • Rat hippocampal membranes and lysates of HEK293 cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.08% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab16718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
IP Use a concentration of 1 - 10 µg/ml.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 73 kDa. Detects a band of approximately 76 KDa in HEK293 lysate and 90 kDa in rat hippocampal membranes.

Target

  • FunctionMediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.
  • Sequence similaritiesBelongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.4/KCNA4 sub-subfamily.
  • DomainThe N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Voltage gated K+ channel HuKII antibody
    • Fetal skeletal muscle potassium channel antibody
    • HBK4 antibody
    • HK1 antibody
    • HPCN2 antibody
    • HUKII antibody
    • Kcna4 antibody
    • KCNA4_HUMAN antibody
    • KCNA4L antibody
    • KCNA8 antibody
    • kv1.4 antibody
    • PCN2 antibody
    • Potassium channel 2 antibody
    • Potassium voltage gated channel shaker related subfamily member 4 antibody
    • Potassium voltage gated channel subfamily A member 4 antibody
    • Potassium voltage-gated channel subfamily A member 4 antibody
    • Rapidly inactivating potassium channel antibody
    • Shaker related potassium channel Kv1.4 antibody
    • Type A potassium channel antibody
    • Voltage gated potassium channel HBK4 antibody
    • Voltage gated potassium channel HK1 antibody
    • Voltage gated potassium channel subunit Kv1.4 antibody
    • Voltage-gated K(+) channel HuKII antibody
    • Voltage-gated potassium channel HBK4 antibody
    • Voltage-gated potassium channel HK1 antibody
    • Voltage-gated potassium channel subunit Kv1.4 antibody
    see all

Anti-Kv1.4 antibody images

  • Western blots using ab16718.

    1: HEK293 cells transfected with Kv1.4.

    2: Rat hippocampal membrane.

    The size difference observed can probably be explained by differential post-translational processing.

  • ab16718 detecting rat recombinant protein by ELISA. Kv1.4 amino terminal domain was immobilized on 96-well plate using streptavidin-biotin interaction. Wells were blocked in 1% milk for 30 minutes at 19°C. The primary antibody was diluted 1/500 & 1/750 in PBS-Tween(0.1%) and incubated for 1 hour at 19°C. HRP conjugated goat monoclonal to rabbit, 1/2000 dilution was used as secondary.

    See Abreview

References for Anti-Kv1.4 antibody (ab16718)

ab16718 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Rat Recombinant protein (E.Coli)
Specification E.Coli
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 19°C
Type Indirect
Username

Abcam user community

Verified customer

Submitted Nov 18 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Recombinant protein (E.Coli)
Loading amount 0.1 µg
Specification E.Coli
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Aug 27 2008

Thank you for your enquiry. I apologize for the delay in finding out this information, I can now inform you that this antibody was raised against a peptide which was conjugated to KLH for immunization. I hope this information will be useful, please...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"