Overview

  • Product name
  • Description
    Rabbit polyclonal to Kv1.6
  • Specificity
    In human HEK293 cells ab21089 recognised a band of ~58 kDa, the predicted MW of Kv1.6 (data not shown). This band was not blocked by the immunising peptide. Additional bands of 100 kDa, 25 kDa and 20 kDa were also recognised.
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-FoFr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human Kv1.6.

    (Peptide available as ab23431.)

  • Positive control
    • This antibody gave a positive signal in Hek293 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab21089 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
IHC-FoFr 1/3000.
IHC-P 1/40. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

Target

  • Function
    Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.
  • Sequence similarities
    Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.6/KCNA6 sub-subfamily.
  • Domain
    The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • HBK 2 antibody
    • HBK2 antibody
    • Human brain potassium channel 2 antibody
    • KCNA 6 antibody
    • Kcna6 antibody
    • KCNA6_HUMAN antibody
    • Potassium voltage gated channel shaker related subfamily member 6 antibody
    • Potassium voltage gated channel subfamily A member 6 antibody
    • Potassium voltage-gated channel subfamily A member 6 antibody
    • Voltage gated potassium channel protein Kv1.6 antibody
    • Voltage gated potassium channel subunit Kv1.6 antibody
    • Voltage-gated potassium channel HBK2 antibody
    • Voltage-gated potassium channel subunit Kv1.6 antibody
    see all

Images

  • All lanes : Anti-Kv1.6 antibody (ab21089) at 1 µg/ml

    Lane 1 : Mouse Brain Lysate
    Lane 2 : Mouse Brain Lysate with Human Kv1.6 peptide (ab23431) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lane 1 : Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
    Lane 2 : Alexa Fluor Goat polyclonal to Rabbit IgG


    Predicted band size : 58 kDa

    ab21089 recognises a band corresponding to the predicted MW of Kv1.6 in mouse brain lysate (lane 1). Antibody binding to this band may be blocked using the immunising peptide, ab23431 (lane 2). The antibody also recognises additional proteins. These may be degradation/cleavage products of Kv1.6, or they may represent non-specific binding by the antibody.

  • ICC/IF image of ab21089 stained human HEK 293 cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab21089, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • Image courtesy of Human Protein Atlas

    ab21089 staining Kv6.1 in human pancreas tissue. Paraffin embedded human pancreas tissue was incubated with ab21089 (1/40 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab21089 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

    .

  • The IHC-FoFr image stainig Kv1.6(ab21089) on Rat DRG sections. The sections used came from animals perfused fixed with Paraformaldehyde 4%, in phosphate buffer 0.2M. Following postfixation in the same fixative overnight, the tissues were cryoprotected in sucrose 30% overnight. Tissues were then cut using a cryostat.

    See Abreview

References

ab21089 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (DRG)
Specification
DRG
Fixative
Paraformaldehyde
Username

Dr. Sophie Pezet

Verified customer

Submitted Sep 06 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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