Overview

  • Product name
    Anti-Kv1.8 antibody
  • Description
    Rabbit polyclonal to Kv1.8
  • Specificity
    ab28724 recognises KCNA10.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide: EDEGFIKDPE TLLPTNDIHR QFWLLFEYPE SSSAARAVAV VSVLVVVISI, corresponding to a region within internal sequence amino acids 181-230 of Human KCNA10

  • Positive control
    • Jurkat cell lysate Human kidney slide

Properties

Applications

Our Abpromise guarantee covers the use of ab28724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 58 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Mediates voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient. The channel activity is up-regulated by cAMP.
  • Tissue specificity
    Detected in kidney, in proximal tubules, glomerular endothelium, in vascular endothelium and in smooth muscle cells.
  • Sequence similarities
    Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.8/KCNA10 sub-subfamily.
  • Domain
    The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cyclic GMP gated potassium channel antibody
    • KCA10_HUMAN antibody
    • Kcn 1 antibody
    • Kcn1 antibody
    • KCNA 10 antibody
    • KCNA10 antibody
    • Kv1.8 antibody
    • Potassium voltage gated channel shaker related subfamily member 10 antibody
    • Potassium voltage gated channel subfamily A member 10 antibody
    • Potassium voltage-gated channel subfamily A member 10 antibody
    • Voltage gated potassium channel subunit Kv1.8 antibody
    • Voltage-gated potassium channel subunit Kv1.8 antibody
    see all

Images

  • Anti-Kv1.8 antibody (ab28724) at 1.25 µg/ml + Jurkat cell lysate

    Secondary
    HRP conjugated anti Rabbit Ig

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
  • ab28724 at 6.0 µg/ml, staining human KCNA10 in paraffin embedded kidney tissue. Arrows indicate positively labelled cells (epithelial cells of the renal tube). Magnification 400X.

References

This product has been referenced in:
  • Lee SI  et al. A null mutation of mouse Kcna10 causes significant vestibular and mild hearing dysfunction. Hear Res 300:1-9 (2013). Read more (PubMed: 23528307) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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