Overview

  • Product nameAnti-Kv2.1 antibody
    See all Kv2.1 primary antibodies
  • Description
    Rabbit polyclonal to Kv2.1
  • Specificityab111122 detects endogenous levels of total Kv2.1 protein.
  • Tested applicationsSuitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from an internal sequence of Human Kv2.1.

  • Positive control
    • COS7 cells; Human brain tissue

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.88% Sodium chloride, 49.1% PBS
    Note: PBS is without Mg2+ and Ca2+
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab111122 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab111122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/500.

Target

  • FunctionMediates the voltage-dependent potassium ion permeability of excitable membranes. Channels open or close in response to the voltage difference across the membrane, letting potassium ions pass in accordance with their electrochemical gradient.
  • Sequence similaritiesBelongs to the potassium channel family. B (Shab) (TC 1.A.1.2) subfamily. Kv2.1/KCNB1 sub-subfamily.
  • DomainThe segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
    The tail may be important in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
  • Post-translational
    modifications
    Highly phosphorylated on serine residues in the C-terminal. Differential phosphorylation on a subset of serines allows graded activity-dependent regulation of channel gating. Phosphorylation on Ser-457, Ser-541, Ser-567, Ser-607, Ser-656 and Ser-720 as well as the N-terminal Ser-15 are all regulated by calcineurin-mediated dephosphorylation. Particularly, Ser-607 and Tyr-128 are significant sites of voltage-gated regulation through phosphorylation/ dephosphorylation activities. Tyr-128 can be dephosphorylated by PTPalpha and cyt-PTPepsilon. Phosphorylation levels on Ser-607 are supersensitive to neuronal activity. Phosphorylation on Ser-567 is reduced during postnatal development with low levels at P2 and P5. Levels then increase to reach adult levels by P14. Phosphorylation levels on Ser-564 and Ser-607 are greatly reduced during seizures, by 40% and 85% respectively.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Delayed rectifier potassium channel 1 antibody
    • Delayed rectifier potassium channel Kv2.1 antibody
    • DRK 1 antibody
    • DRK1 antibody
    • h DRK1 K(+) channel antibody
    • h-DRK1 antibody
    • hDRK 1 antibody
    • hDRK1 antibody
    • KCB 1 antibody
    • KCB1 antibody
    • KCNB1 antibody
    • KCNB1_HUMAN antibody
    • KV2.1 antibody
    • Potassium channel protein DRK1 antibody
    • Potassium voltage gated channel shab related subfamily member 1 antibody
    • Potassium voltage-gated channel subfamily B member 1 antibody
    • Voltage-gated potassium channel subunit Kv2.1 antibody
    see all

Anti-Kv2.1 antibody images

  • Immunofluorescence analysis of Kv2.1 in COS7 cells, using ab111122 at a 1/100 dilution. The image on the right is from cells treated with the synthesized peptide.
  • ab111122 at 1/50 dilution staining Kv2.1 in paraffin embedded Human brain tissue by Immunohistochemistry. The image on the right is from cells treated with the synthesized peptide.

References for Anti-Kv2.1 antibody (ab111122)

This product has been referenced in:
  • Kim SH  et al. Electrogenic transport and K(+) ion channel expression by the human endolymphatic sac epithelium. Sci Rep 5:18110 (2015). IHC ; Human . Read more (PubMed: 26655723) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab111122.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"