Anti-Kv2.1 (phospho S805) antibody (ab111552)

Overview

  • Product name
    Anti-Kv2.1 (phospho S805) antibody
    See all Kv2.1 primary antibodies
  • Description
    Rabbit polyclonal to Kv2.1 (phospho S805)
  • Specificity
    ab111552 detects endogenous levels of Kv2.1 only when phosphorylated at serine 805 (Human: Ser805; Mouse: Ser804; Rat: Ser804).
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from Human Kv2.1 around the phosphorylation site of Serine 805 (P-T-SP-P-K).

  • Positive control
    • Extracts from K562 cells treated with TNF (200ng/ml 30mins)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 49% PBS, 0.88% Sodium chloride
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab111552 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab111552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 96 kDa.

Target

  • Function
    Mediates the voltage-dependent potassium ion permeability of excitable membranes. Channels open or close in response to the voltage difference across the membrane, letting potassium ions pass in accordance with their electrochemical gradient.
  • Sequence similarities
    Belongs to the potassium channel family. B (Shab) (TC 1.A.1.2) subfamily. Kv2.1/KCNB1 sub-subfamily.
  • Domain
    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
    The tail may be important in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
  • Post-translational
    modifications
    Highly phosphorylated on serine residues in the C-terminal. Differential phosphorylation on a subset of serines allows graded activity-dependent regulation of channel gating. Phosphorylation on Ser-457, Ser-541, Ser-567, Ser-607, Ser-656 and Ser-720 as well as the N-terminal Ser-15 are all regulated by calcineurin-mediated dephosphorylation. Particularly, Ser-607 and Tyr-128 are significant sites of voltage-gated regulation through phosphorylation/ dephosphorylation activities. Tyr-128 can be dephosphorylated by PTPalpha and cyt-PTPepsilon. Phosphorylation levels on Ser-607 are supersensitive to neuronal activity. Phosphorylation on Ser-567 is reduced during postnatal development with low levels at P2 and P5. Levels then increase to reach adult levels by P14. Phosphorylation levels on Ser-564 and Ser-607 are greatly reduced during seizures, by 40% and 85% respectively.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Delayed rectifier potassium channel 1 antibody
    • Delayed rectifier potassium channel Kv2.1 antibody
    • DRK 1 antibody
    • DRK1 antibody
    • h DRK1 K(+) channel antibody
    • h-DRK1 antibody
    • hDRK 1 antibody
    • hDRK1 antibody
    • KCB 1 antibody
    • KCB1 antibody
    • KCNB1 antibody
    • KCNB1_HUMAN antibody
    • KV2.1 antibody
    • Potassium channel protein DRK1 antibody
    • Potassium voltage gated channel shab related subfamily member 1 antibody
    • Potassium voltage-gated channel subfamily B member 1 antibody
    • Voltage-gated potassium channel subunit Kv2.1 antibody
    see all

Anti-Kv2.1 (phospho S805) antibody images

  • All lanes : Anti-Kv2.1 (phospho S805) antibody (ab111552) at 1/500 dilution

    Lane 1 : Extracts from K562 cells treated with TNF (200ng/ml 30mins)
    Lane 2 : Extracts from K562 cells treated with TNF (200ng/ml 30mins) with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 96 kDa

References for Anti-Kv2.1 (phospho S805) antibody (ab111552)

ab111552 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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