This antibody gave a positive signal in Mouse and Rat Heart tissue lysates.
This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: SKNSH
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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 70 kDa).
Use a concentration of 5 µg/ml.
Pore-forming (alpha) subunit of voltage-gated rapidly inactivating A-type potassium channels. May contribute to I(To) current in heart and I(Sa) current in neurons. Channel properties are modulated by interactions with other alpha subunits and with regulatory subunits.
Highly expressed throughout the brain. Expression is very low or absent in other tissues.
Belongs to the potassium channel family. D (Shal) (TC 1.A.1.2) subfamily. Kv4.2/KCND2 sub-subfamily.
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
Phosphorylated on serine and threonine residues.
Cell membrane. Cell projection > dendrite. Detected in dendrites in cultured hippocampal neurons. Association with KCNIP2 probably enhances cell surface expression.
ab123543 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab123543 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.