Overview

  • Product name
  • Description
    Rabbit polyclonal to Kv4.2
  • Tested applications
    Suitable for: WB, ICC/IF, IP, ELISAmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Rabbit, Human, Ferret
  • Immunogen

    Synthetic peptide:

    ASGPMPAPPRQERKRTQDALI

    conjugated to KLH, corresponding to amino acids 23-43 of Rat Kv4.2.

  • Positive control
    • Transfected HEK293 cells containing Kv4.2 cDNA.

Properties

Applications

Our Abpromise guarantee covers the use of ab16719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 70 kDa).
ICC/IF Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.

Target

  • Function
    Pore-forming (alpha) subunit of voltage-gated rapidly inactivating A-type potassium channels. May contribute to I(To) current in heart and I(Sa) current in neurons. Channel properties are modulated by interactions with other alpha subunits and with regulatory subunits.
  • Tissue specificity
    Highly expressed throughout the brain. Expression is very low or absent in other tissues.
  • Sequence similarities
    Belongs to the potassium channel family. D (Shal) (TC 1.A.1.2) subfamily. Kv4.2/KCND2 sub-subfamily.
  • Domain
    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
  • Post-translational
    modifications
    Phosphorylated on serine and threonine residues.
  • Cellular localization
    Cell membrane. Cell projection > dendrite. Detected in dendrites in cultured hippocampal neurons. Association with KCNIP2 probably enhances cell surface expression.
  • Information by UniProt
  • Database links
  • Alternative names
    • KCD2 antibody
    • KCND 2 antibody
    • KCND2 antibody
    • KCND2_HUMAN antibody
    • KIAA1044 antibody
    • MGC119702 antibody
    • MGC119703 antibody
    • Potassium voltage gated channel Shal related subfamily member 2 antibody
    • Potassium voltage-gated channel subfamily D member 2 antibody
    • RK 5 antibody
    • RK5 antibody
    • Voltage gated potassium channel Kv4.2 antibody
    • Voltage gated potassium channel subunit Kv4.2 antibody
    • Voltage sensitive potassium channel antibody
    • Voltage-gated potassium channel subunit Kv4.2 antibody
    see all

Anti-Kv4.2 antibody images

  • ab16719 detecting Kv4.2 by ELISA. The sample was purified from rat neuronal cells and blocked with 1% milk for 1 hour at 18°C. The primary antibody was used at 1/1000 dilution, and incubated with sample for 1 hour at 18°C.       

    See Abreview

  • All lanes : Anti-Kv4.2 antibody (ab16719)

    Lane 1 : Lysate prepared from total cellular protein (intracellular + surface)
    Lane 2 : Lysate prepared from total cellular protein (intracellular + surface)
    Lane 3 : Lysate prepared from total cellular protein (intracellular + surface)
    Lane 4 : Lysate prepared from biotinylated, surface-expressed channels (as supported by the absence of the intracellular protein synaptophysin)
    Lane 5 : Lysate prepared from biotinylated, surface-expressed channels (as supported by the absence of the intracellular protein synaptophysin)
    Lane 6 : Lysate prepared from biotinylated, surface-expressed channels (as supported by the absence of the intracellular protein synaptophysin)


    Predicted band size : 70 kDa

    Image from Noam Y et al, J Biol Chem. 2010 May 7;285(19):14724-36. Epub 2010 Mar 9, Fig 8.

References for Anti-Kv4.2 antibody (ab16719)

This product has been referenced in:
  • Yang CH  et al. A critical role for protein tyrosine phosphatase nonreceptor type 5 in determining individual susceptibility to develop stress-related cognitive and morphological changes. J Neurosci 32:7550-62 (2012). Read more (PubMed: 22649233) »
  • Noam Y  et al. Trafficking and surface expression of hyperpolarization-activated cyclic nucleotide-gated channels in hippocampal neurons. J Biol Chem 285:14724-36 (2010). WB ; Rat . Read more (PubMed: 20215108) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Rat Purified protein (neuronal)
Specification
neuronal
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 18°C
Type
Indirect
Username

Abcam user community

Verified customer

Submitted May 06 2009

Application
Western blot
Sample
Escherichia coli Purified protein (E.Coli)
Loading amount
0.05 µg
Specification
E.Coli
Gel Running Conditions
Reduced Denaturing (gradient 4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jan 09 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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