Overview

  • Product nameAnti-Kv4.2 antibody [S57-1]
    See all Kv4.2 primary antibodies
  • Description
    Mouse monoclonal [S57-1] to Kv4.2
  • SpecificityNo cross-reactivity is observed against Kv4.3
  • Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-P, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Rabbit, Human, Pig, Orangutan
  • Immunogen

    Synthetic peptide:

    CGSSPGHIKELPSGERY

    , corresponding to extracellular S1-S2 loop amino acids 209 - 225 of Rat Kv4.2 (accession number Q63881).

  • Positive control
    • Rat brain lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab99040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 0.1 - 1 µg/ml. (perox), 1 - 10µg/mL (IF).
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 71 kDa.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 0.1 - 1 µg/ml. (perox), 1 - 10µg/mL (IF).
IHC-Fr Use a concentration of 0.1 - 1 µg/ml. (perox), 1 - 10µg/mL (IF).
Flow Cyt Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionPore-forming (alpha) subunit of voltage-gated rapidly inactivating A-type potassium channels. May contribute to I(To) current in heart and I(Sa) current in neurons. Channel properties are modulated by interactions with other alpha subunits and with regulatory subunits.
  • Tissue specificityHighly expressed throughout the brain. Expression is very low or absent in other tissues.
  • Sequence similaritiesBelongs to the potassium channel family. D (Shal) (TC 1.A.1.2) subfamily. Kv4.2/KCND2 sub-subfamily.
  • DomainThe segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
  • Post-translational
    modifications
    Phosphorylated on serine and threonine residues.
  • Cellular localizationCell membrane. Cell projection > dendrite. Detected in dendrites in cultured hippocampal neurons. Association with KCNIP2 probably enhances cell surface expression.
  • Information by UniProt
  • Database links
  • Alternative names
    • KCD2 antibody
    • KCND 2 antibody
    • KCND2 antibody
    • KCND2_HUMAN antibody
    • KIAA1044 antibody
    • MGC119702 antibody
    • MGC119703 antibody
    • Potassium voltage gated channel Shal related subfamily member 2 antibody
    • Potassium voltage-gated channel subfamily D member 2 antibody
    • RK 5 antibody
    • RK5 antibody
    • Voltage gated potassium channel Kv4.2 antibody
    • Voltage gated potassium channel subunit Kv4.2 antibody
    • Voltage sensitive potassium channel antibody
    • Voltage-gated potassium channel subunit Kv4.2 antibody
    see all

Anti-Kv4.2 antibody [S57-1] images

  • ab99040 staining Kv4.2 in Mouse brain tissue sections by Immunohistochemistry.

  • ab99040 staining Kv4.2 in Mouse cerebellum tissue sections by Immunohistochemistry.

  • Anti-Kv4.2 antibody [S57-1] (ab99040) at 1/1000 dilution + Rat brain membrane tissue lysate

    Predicted band size : 71 kDa
  • Overlay histogram showing SH-SY5Y cells stained with ab99040 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab99040, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-Kv4.2 antibody [S57-1] (ab99040)

This product has been referenced in:
  • Gaborit N  et al. Cooperative and antagonistic roles for Irx3 and Irx5 in cardiac morphogenesis and postnatal physiology. Development 139:4007-19 (2012). ICC/IF ; Mouse . Read more (PubMed: 22992950) »

See 1 Publication for this product

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