Overview

  • Product nameAnti-Kv4.3 antibody
    See all Kv4.3 primary antibodies
  • Description
    Rabbit polyclonal to Kv4.3
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Pig
  • Immunogen

    Synthetic peptide: AKTGSSNAYL HSKRNGLLNE ALELTGTPEE EHMGKTTSLI ESQHHHLLHC, corresponding to intracellular amino acids 433-482 of Human Kv4.3

  • Positive control
    • HepG2 lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab49348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:12500.

WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 61, 42 kDa (predicted molecular weight: 70 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionPore-forming (alpha) subunit of voltage-gated rapidly inactivating A-type potassium channels. May contribute to I(To) current in heart and I(Sa) current in neurons. Channel properties are modulated by interactions with other alpha subunits and with regulatory subunits.
  • Tissue specificityHighly expressed in heart and brain, in particular in cortex, cerebellum, amygdala and caudate nucleus. Detected at lower levels in liver, skeletal muscle, kidney and pancreas. Isoform 1 predominates in most tissues. Isoform 1 and isoform 2 are detected at similar levels in brain, skeletal muscle and pancreas.
  • Sequence similaritiesBelongs to the potassium channel family. D (Shal) (TC 1.A.1.2) subfamily. Kv4.3/KCND3 sub-subfamily.
  • DomainThe segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
  • Cellular localizationMembrane. Cell membrane > sarcolemma.
  • Information by UniProt
  • Database links
  • Alternative names
    • KCND 3 antibody
    • KCND 3L antibody
    • KCND 3S antibody
    • Kcnd3 antibody
    • KCND3_HUMAN antibody
    • KCND3L antibody
    • KCND3S antibody
    • KSHIVB antibody
    • Kv 4.3 antibody
    • MGC14203 antibody
    • MGC142037 antibody
    • OTTHUMP00000063948 antibody
    • Potassium ioinic channel Kv4.3 antibody
    • Potassium voltage gated channel Shal related subfamily member 3 antibody
    • Potassium voltage gated channel subfamily D member 3 antibody
    • Potassium voltage-gated channel subfamily D member 3 antibody
    • Sha1 related potassium channel Kv4.3 antibody
    • Voltage gated K+ channel antibody
    • Voltage gated potassium channel Kv4.3 antibody
    • Voltage gated potassium channel subunit Kv4.3 antibody
    • Voltage-gated potassium channel subunit Kv4.3 antibody
    see all

Anti-Kv4.3 antibody images

  • Anti-Kv4.3 antibody (ab49348) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG 1/50,000 -1/100,000

    Predicted band size : 70 kDa
    Observed band size : 60,61 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 42 kDa (possible non-specific binding).

References for Anti-Kv4.3 antibody (ab49348)

ab49348 has not yet been referenced specifically in any publications.

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