Anti-kynurenine 3-monooxygenase antibody (ab83929)

Overview

  • Product name
    Anti-kynurenine 3-monooxygenase antibody
    See all kynurenine 3-monooxygenase primary antibodies
  • Description
    Rabbit polyclonal to kynurenine 3-monooxygenase
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide, within internal sequence amino acids 200-299 of Mouse kynurenine 3-monooxygenase

  • Positive control
    • Mouse brain extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab83929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
ELISA Use at an assay dependent dilution.

Target

  • Function
    Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid, a neurotoxic NMDA receptor antagonist and potential endogenous inhibitor of NMDA receptor signaling in axonal targeting, synaptogenesis and apoptosis during brain development. Quinolinic acid may also affect NMDA receptor signaling in pancreatic beta cells, osteoblasts, myocardial cells, and the gastrointestinal tract.
  • Tissue specificity
    Highest levels in placenta and liver. Detectable in kidney.
  • Pathway
    Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 1/3.
  • Sequence similarities
    Belongs to the aromatic-ring hydroxylase family. KMO subfamily.
  • Cellular localization
    Mitochondrion outer membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • dJ317G22.1 antibody
    • kmo antibody
    • KMO_HUMAN antibody
    • Kynurenine 3 hydroxylase antibody
    • Kynurenine 3 monooxygenase (kynurenine 3 hydroxylase) antibody
    • Kynurenine 3 monooxygenase antibody
    • Kynurenine 3-hydroxylase antibody
    • Kynurenine 3-monooxygenase antibody
    • RP1-317G22.1 antibody
    see all

Anti-kynurenine 3-monooxygenase antibody images

  • Anti-kynurenine 3-monooxygenase antibody (ab83929) at 1 µg/ml + Mouse brain extract

    Predicted band size : 56 kDa
    Observed band size : 56 kDa

References for Anti-kynurenine 3-monooxygenase antibody (ab83929)

This product has been referenced in:
  • Nagy-Grócz G  et al. The Effect of Systemic Nitroglycerin Administration on the Kynurenine Pathway in the Rat. Front Neurol 8:278 (2017). WB ; Rat . Read more (PubMed: 28659861) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Purified protein (Brain, Hyp and Hpc)
Loading amount
30 µg
Specification
Brain, Hyp and Hpc
Treatment
Prenatal stress paradigm
Gel Running Conditions
Reduced Denaturing (7.5% TrisHCl)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Apr 23 2013

Thank you for the update. Our datasheet shows a nice clean blot of mouse brain, so it is frustrating that you do not get that result with your brain samples. The placenta result may be real but given the other bands in that blot, I would be skeptical. ...

Read More

Thank you for your enquiry.

I have obtained some further information regarding the WB image on the datasheet.

The sample were extracts of mouse brain, each lane represents approximately 20 ug of extract. The mouse brain extract wa...

Read More

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