Overview

  • Product nameAnti-l Afadin antibody
    See all l Afadin primary antibodies
  • Description
    Rabbit polyclonal to l Afadin
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Saccharomyces cerevisiae, Drosophila melanogaster, Zebrafish
  • Immunogen

    A 14-22 amino acid region within synthetic peptide: EKQEKEGVIQ NFKRTLSKKE KKEKKKREKE ALRQASDKDD RPFQGEDVEN, corresponding to N terminal amino acids 145-194 of Human l Afadin

  • Positive control
    • HepG2 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab62268 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 206 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionBelongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton.
  • Involvement in diseaseNote=A chromosomal aberration involving MLLT4 is associated with acute leukemias. Translocation t(6;11)(q27;q23) with MLL/HRX. The result is a rogue activator protein.
  • Sequence similaritiesContains 1 dilute domain.
    Contains 1 FHA domain.
    Contains 1 PDZ (DHR) domain.
    Contains 2 Ras-associating domains.
  • DomainThe PDZ/DHR domain interacts with the C-terminus of nectin and the Pro-rich N-terminus domain interacts with F-actin.
  • Cellular localizationCell junction > adherens junction. Not found at cell-matrix AJs.
  • Information by UniProt
  • Database links
  • Alternative names
    • AF6 antibody
    • AFAD_HUMAN antibody
    • Afadin antibody
    • ALL1-fused gene from chromosome 6 protein antibody
    • Mllt4 antibody
    • Protein Af-6 antibody
    • Protein AF6 antibody
    see all

Anti-l Afadin antibody images

  • All lanes : l Afadin antibody (ab62268) at 1.25µg/ml (in 5% skim milk / PBS buffer)

    Lane 1 : Molecular weight marker
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 206 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)

References for Anti-l Afadin antibody (ab62268)

ab62268 has not yet been referenced specifically in any publications.

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