Overview

  • Product nameL-Alanine Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Sensitivity
    > 24 µg/ml
  • Range
    24 µg/ml - 76 µg/ml
  • Assay time
    0h 40m
  • Product overview

    Abcam's Alanine Assay Kit provides a sensitive detection method of alanine. In the kit, alanine is converted to pyruvate which is specifically detected leading to proportional color (?=570nm: 0-10 nmol) or fluorescence (Ex/Em 535/587nm: 0-1 nmol) development. Serum concentration: ~24-76 µg/ml (~3-9 nmol/10 µl).
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Alanine is the 2nd most abundant of the 20 proteinogenic amino acids. Nonessential, being available from dietary sources, it plays a key role in the glucose-alanine cycle between tissues and liver. In muscle and other tissues that degrade amino acids, amino groups are pooled as glutamate by transamination. Glutamate then transfers the amino group to pyruvate via alanine aminotransferase, forming alanine and a-ketoglutarate. The alanine is passed into the blood and transported to the liver. A reverse of the alanine aminotransferase reaction takes place in liver. Pyruvate can be used in gluconeogenesis, to form glucose which may return to other tissues through the circulatory system. There appears to be a correlation between alanine levels and and higher blood pressure, energy intake, cholesterol levels, and body mass index.

  • Tested applicationsSuitable for: Functional Studiesmore details

Properties

  • RelevanceAlanine is the second most abundant of the 20 proteinogenic amino acids. Nonessential, being available from dietary sources, it plays a key role in the glucose-alanine cycle between tissues and liver. In muscle and other tissues that degrade amino acids, amino groups are pooled as glutamate by transamination. Glutamate then transfers the amino group to pyruvate via alanine aminotransferase, forming alanine and alpha ketoglutarate. The alanine is passed into the blood and transported to the liver. A reverse of the alanine aminotransferase reaction takes place in liver. Pyruvate can be used in gluconeogenesis, to form glucose which may return to other tissues through the circulatory system. There appears to be a correlation between alanine levels and and higher blood pressure, energy intake, cholesterol levels, and body mass index.
  • Alternative names
    • Ala

Applications

Our Abpromise guarantee covers the use of ab83394 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

L-Alanine Assay Kit images

  • L-Alanine measured fluorometrically in cell culture medium and control medium, background signal subtracted (duplicates +/- SD).

  • L-Alanine measured fluorometrically in cell lysates, background signal subtracted (duplicates +/- SD).

  • L-Alanine measured fluorometrically in deproteinised human biological fluid with background signal subtracted (duplicates +/- SD).

  • L-Alanine measured colourimetrically in cell culture medium and control medium, background signal subtracted (duplicates +/- SD).

  • L-Alanine measured colourimetrically in deproteinised human biological fluid with background signal subtracted (duplicates +/- SD).

  • Example of fluorometric and colorimetric Alanine standard curves obtained using ab83394.

Protocols

References for L-Alanine Assay Kit (ab83394)

ab83394 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us yesterday in regards to the Alanine Transaminase Activity Assay Kit (ab105134). I have now had a chance to have a look at this product to see if it would be suitable to use in your experiment to assess the transaminase inhib...

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Thank you for contacting us. I have contacted the lab regarding this for you. The product does contain alanine aminotransferase (ALT). I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or ...

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Thank you for contacting us. I have spoken with the lab regarding the homogenization and wait peroid for use with this kit. They usually use a hand held dounce homogenizer, but pipetting back and forth in Assay buffer (it contains detergent) along w...

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* Generally, the usual mistake is that the probe is still frozen DMSO. Please make sure to warm the probe for 1-2 min in a 37 °C bath, pipette up/down to ensure probe is fully melted, then repeat the standard curve. Please send us the data if problem p...

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All of the kits: ab65337, ab65342, ab83390, ab83394 are suitable for cell and tissue culture supernatants. As well as many other biological fluids like urine, plasma and serum.

Thank you for your inquiry. We generally recommend to lyse cells in the kits own assay buffer, as it is formulated with components conducive for the optimum reaction to occur. I hope this information helps. Please contact me with any other quest...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"