L-Alanine Assay Kit (ab83394)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameL-Alanine Assay Kit
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Sample typeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
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Assay typeQuantitative
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Sensitivity> 24 µg/ml
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Range24 µg/ml - 76 µg/ml
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Assay time0h 40m
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Product overview
Abcam's Alanine Assay Kit provides a sensitive detection method of alanine. In the kit, alanine is converted to pyruvate which is specifically detected leading to proportional color (?=570nm: 0-10 nmol) or fluorescence (Ex/Em 535/587nm: 0-1 nmol) development. Serum concentration: ~24-76 µg/ml (~3-9 nmol/10 µl).
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Notes
Alanine is the 2nd most abundant of the 20 proteinogenic amino acids. Nonessential, being available from dietary sources, it plays a key role in the glucose-alanine cycle between tissues and liver. In muscle and other tissues that degrade amino acids, amino groups are pooled as glutamate by transamination. Glutamate then transfers the amino group to pyruvate via alanine aminotransferase, forming alanine and a-ketoglutarate. The alanine is passed into the blood and transported to the liver. A reverse of the alanine aminotransferase reaction takes place in liver. Pyruvate can be used in gluconeogenesis, to form glucose which may return to other tissues through the circulatory system. There appears to be a correlation between alanine levels and and higher blood pressure, energy intake, cholesterol levels, and body mass index.
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Tested applicationsSuitable for: Functional Studiesmore details
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Components Identifier 100 tests Alanine Assay Buffer WM 1 x 25ml Alanine Converting Enzyme (Lyophilised) Purple 1 vial Alanine Development Mix (Lyophilised) Green 1 vial Alanine probe (in DMSO) Red 1 x 200µl Alanine Standard (10 µmol) (Lyophilised) Yellow 1 vial -
Research areas
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RelevanceAlanine is the second most abundant of the 20 proteinogenic amino acids. Nonessential, being available from dietary sources, it plays a key role in the glucose-alanine cycle between tissues and liver. In muscle and other tissues that degrade amino acids, amino groups are pooled as glutamate by transamination. Glutamate then transfers the amino group to pyruvate via alanine aminotransferase, forming alanine and alpha ketoglutarate. The alanine is passed into the blood and transported to the liver. A reverse of the alanine aminotransferase reaction takes place in liver. Pyruvate can be used in gluconeogenesis, to form glucose which may return to other tissues through the circulatory system. There appears to be a correlation between alanine levels and and higher blood pressure, energy intake, cholesterol levels, and body mass index.
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Alternative names
- Ala
Applications
Our Abpromise guarantee covers the use of ab83394 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies | Use at an assay dependent dilution. |
Images
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Functional Studies – L-Alanine Assay Kit (ab83394)L-Alanine measured fluorometrically in cell culture medium and control medium, background signal subtracted (duplicates +/- SD).
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Functional Studies – L-Alanine Assay Kit (ab83394)L-Alanine measured fluorometrically in cell lysates, background signal subtracted (duplicates +/- SD).
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Functional Studies – L-Alanine Assay Kit (ab83394)L-Alanine measured fluorometrically in deproteinised human biological fluid with background signal subtracted (duplicates +/- SD).
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Functional Studies – L-Alanine Assay Kit (ab83394)L-Alanine measured colourimetrically in cell culture medium and control medium, background signal subtracted (duplicates +/- SD).
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Functional Studies – L-Alanine Assay Kit (ab83394)L-Alanine measured colourimetrically in deproteinised human biological fluid with background signal subtracted (duplicates +/- SD).
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Functional Studies - L-Alanine Assay Kit (ab83394)Example of fluorometric and colorimetric Alanine standard curves obtained using ab83394.
Protocols
References
This product has been referenced in:
- McGivney E et al. Disruption of Autolysis in Bacillus subtilis using TiO2 Nanoparticles. Sci Rep 7:44308 (2017). Read more (PubMed: 28303908) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"