Abcam's Alanine Assay Kit provides a sensitive detection method of alanine. In the kit, alanine is converted to pyruvate which is specifically detected leading to proportional color (?=570nm: 0-10 nmol) or fluorescence (Ex/Em 535/587nm: 0-1 nmol) development. Serum concentration: ~24-76 µg/ml (~3-9 nmol/10 µl).
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Alanine is the 2nd most abundant of the 20 proteinogenic amino acids. Nonessential, being available from dietary sources, it plays a key role in the glucose-alanine cycle between tissues and liver. In muscle and other tissues that degrade amino acids, amino groups are pooled as glutamate by transamination. Glutamate then transfers the amino group to pyruvate via alanine aminotransferase, forming alanine and a-ketoglutarate. The alanine is passed into the blood and transported to the liver. A reverse of the alanine aminotransferase reaction takes place in liver. Pyruvate can be used in gluconeogenesis, to form glucose which may return to other tissues through the circulatory system. There appears to be a correlation between alanine levels and and higher blood pressure, energy intake, cholesterol levels, and body mass index.
|Alanine Assay Buffer||WM||1 x 25ml|
|Alanine Converting Enzyme (Lyophilised)||Purple||1 vial|
|Alanine Development Mix (Lyophilised)||Green||1 vial|
|Alanine probe (in DMSO)||Red||1 x 200µl|
|Alanine Standard (10 µmol) (Lyophilised)||Yellow||1 vial|
Our Abpromise guarantee covers the use of ab83394 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
L-Alanine measured fluorometrically in cell culture medium and control medium, background signal subtracted (duplicates +/- SD).
L-Alanine measured fluorometrically in cell lysates, background signal subtracted (duplicates +/- SD).
L-Alanine measured fluorometrically in deproteinised human biological fluid with background signal subtracted (duplicates +/- SD).
L-Alanine measured colourimetrically in cell culture medium and control medium, background signal subtracted (duplicates +/- SD).
L-Alanine measured colourimetrically in deproteinised human biological fluid with background signal subtracted (duplicates +/- SD).