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Abcam's L-Amino Acid Assay Kit provides a convenient means for directly detecting L-amino acid in biological samples. There is no requirement for sample pretreatment or purification when using this kit. The L-amino acid(s) level can be quantified using fluorometric (at Ex/Em = 535/587 nm) or colorimetric (at ? = 570 nm) methods in 96-well plates.
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L-Amino acids are the most essential elements in biology. Accurately quantitating L-amino acids in body fluids or purified samples may provide valuable information for diagnostic or basic research studies.
|L-Amino Acid Assay Buffer||WM||1 x 25ml|
|L-Amino Acid Enzyme Mix||Green||1 vial|
|L-amino Acid Probe||Red||1 x 0.2ml|
|L-Amino Acid Standard (4 nmol/µl)||Yellow||1 x 300µl|
Our Abpromise guarantee covers the use of ab65347 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Standard curve (fluorimetrical): mean of duplicates (+/-) with background subtracted
Standard curve (colorimetrical): mean of duplicates (+/-SD) with background readings subtracted