Overview

  • Product nameL-Glutamate
  • Description
    Excitatory neurotransmitter
  • Biological descriptionMajor excitatory neurotransmitter. Acts at glutamate receptors. Active in vivo.
  • Purity> 99%

Properties

    Applications

    Our Abpromise guarantee covers the use of ab120049 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    L-Glutamate images

    • ab55051 staining GABA B receptor 1 in SK-N-SH cells treated with L-Glutamate (ab120049), by ICC/IF. Internalization of GABA B receptor 1 correlates with increased concentration of L-Glutamate, as described in literature.
      The cells were incubated at 37°C for 30 minutes in media containing different concentrations of ab120049 (L-Glutamate) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab55051 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    References for L-Glutamate (ab120049)

    This product has been referenced in:
    • Hainsworth AH  et al. Glutamate receptor-mediated inhibition of L-glutamate efflux from cerebral cortex in vitro. Brain Res 1114:36-40 (2006). Read more (PubMed: 16904087) »
    • Kew JN & Kemp JA Ionotropic and metabotropic glutamate receptor structure and pharmacology. Psychopharmacology (Berl) 179:4-29 (2005). Read more (PubMed: 15731895) »

    See 0 Publications for this product

    Product Wall

    To prepare the 1 gram vial in 1 eq. NaOH, you'll need to solubilize each 14.7 mg of the L-glutamate in 1 mL of 100 mM NaOH (due to batch-specific molecular weight of 147.13 g/mol). So, to prepare the full vial, you'll add 68.03 mL of 100 mM NaOH (10...

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    The answer to this can be found on the biochemicals FAQ section of the PWS. http://www.abcam.com/index.html?pageconfig=resource&rid=14735#NaOH

    If you look under the Solubilisation heading then “How do I make solutions using NaOH equi...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"