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L Glutamate cross-linked to purifed fraction V bovine serum albumin with glutaraldehyde.
Our Abpromise guarantee covers the use of ab62668 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC - Wholemount||Use at an assay dependent concentration.|
|ICC||1/100. Endogenous content mapping by LM and EM immunocytochemistry. True dilution at user dilution: 1/32000. Optimal fixation: 0.1-2.5% glutaraldehyde,1% formaldehyde using HPI (High Performance Immunocytochemistry). Minimum glutaraldehyde: 0.0% using EHPI (Enhanced HPI) with 4% formaldehyde.|
IHC - Wholemount of mouse brain tissue labelling L Glutamate with ab62668. Sample was incubated with primary antibody (1/100 in 1X PBS + 0.3% Triton X-100 + 2 % normal goat serum) for 48 hours at 4°C. ab150174, a goat anti-chicken IgY Alexa Fluor® 555 (1/500) was used as the secondary antibody (assigned red color).
ab62668 has not yet been referenced specifically in any publications.