Anti-l-Myc antibody (ab28739)


  • Product nameAnti-l-Myc antibody
    See all l-Myc primary antibodies
  • Description
    Rabbit polyclonal to l-Myc
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    A region within synthetic peptide: LERAVSDRLA PGAPRGNPPK ASAAPDCTPS LEAGNPAPAA PCPLGEPKTQ, corresponding to internal sequence amino acids 105-154 of Human l-Myc

  • Positive control
    • Human foetal kidney lysate, human kidney tissue.



Our Abpromise guarantee covers the use of ab28739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 2.5 µg/ml. Predicted molecular weight: 40,25 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


Anti-l-Myc antibody images

  • Immunohistochemical staining of paraffin embedded human kidney with ab28739 at 4-8 ug/ml. Arrows indicate postive staining of epithelial cells of the renal tube.
  • Anti-l-Myc antibody (ab28739) at 2.5 µg/ml + Human foetal kidney lysate

    HRP conjugated anti-Rabbit IgG 1: 50,000 - 100,000

    Predicted band size : 40,25 kDa
    Observed band size : 23 kDa (why is the actual band size different from the predicted?)

References for Anti-l-Myc antibody (ab28739)

ab28739 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply. For the c-myc antibody, I suggest that you look at ab56 or ab11917. A positive control would be ab7914. Please let me know which would be best for you. I also hope that you are able to find the replacement l-myc that was...

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Thank you for contacting Abcam.

I am sorry to hear of the continued disappointing results from ab28739. I would be glad to send you a different antibody against l-myc or to issue you a credit note for the purchase. Please let me know what you...

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To my knowledge, customized protocols were not used in for this product. I've linked our standard western blotting protocol here: In our published sample, Anti-l-Myc antibody (ab28739)was us...

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Thank you for contacting us regarding this product. I am sorry that you have been experiencing difficulties using our product and look forward to hearing the results of your positive control experiment. At Abcam, providing quality service is just as...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293 cell)
Loading amount 50 µg
Specification HEK293 cell
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C

Abcam user community

Verified customer

Submitted Aug 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Renal Pelvis of transgenic mouse)
Specification Renal Pelvis of transgenic mouse
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Nov 24 2010