Overview

  • Product name
    Anti-l + S Afadin antibody
    See all l + S Afadin primary antibodies
  • Description
    Rabbit polyclonal to l + S Afadin
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human
  • Immunogen

    Synthetic peptide:

    CKLPGDDRLMKNRADHRS

    conjugated to maleimide-activated BSA, corresponding to amino acids 1163-1179 of Rat l + S Afadin (with N-terminal added cysteine).

  • General notes
    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Properties

Applications

Our Abpromise guarantee covers the use of ab11338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 200 (l), 190 (s) kDa.
ICC/IF Use at an assay dependent dilution.

Target

  • Function
    Belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton.
  • Involvement in disease
    Note=A chromosomal aberration involving MLLT4 is associated with acute leukemias. Translocation t(6;11)(q27;q23) with MLL/HRX. The result is a rogue activator protein.
  • Sequence similarities
    Contains 1 dilute domain.
    Contains 1 FHA domain.
    Contains 1 PDZ (DHR) domain.
    Contains 2 Ras-associating domains.
  • Domain
    The PDZ/DHR domain interacts with the C-terminus of nectin and the Pro-rich C-terminus domain interacts with F-actin.
  • Cellular localization
    Cell junction > adherens junction. Not found at cell-matrix AJs.
  • Information by UniProt
  • Database links
  • Alternative names
    • AF6 antibody
    • AFAD_HUMAN antibody
    • Afadin antibody
    • ALL1-fused gene from chromosome 6 protein antibody
    • Mllt4 antibody
    • Protein Af-6 antibody
    • Protein AF6 antibody
    see all

References

This product has been referenced in:
  • Tanaka-Okamoto M  et al. Genetic ablation of afadin causes mislocalization and deformation of Paneth cells in the mouse small intestinal epithelium. PLoS One 9:e110549 (2014). Read more (PubMed: 25333284) »
  • Lachke SA  et al. The cell adhesion gene PVRL3 is associated with congenital ocular defects. Hum Genet : (2011). IHC-Fr ; Mouse . Read more (PubMed: 21769484) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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