Overview

  • Product nameAnti-l + S Afadin antibody
    See all l + S Afadin primary antibodies
  • Description
    Rabbit polyclonal to l + S Afadin
  • SpecificityAnti l/s Afadin specifically recognizes l afadin and s afadin by immunoblotting (approximately 200 and 190 kDa respectively). Staining of the l/s afadin bands is specifically inhibited by the immunizing peptide.
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human
  • Immunogen

    Synthetic peptide:

    CKLPGDDRLMKNRADHRS

    conjugated to maleimide-activated BSA, corresponding to amino acids 1163-1179 of Rat l + S Afadin (with N-terminal added cysteine).

  • General notesStorage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Properties

Applications

Our Abpromise guarantee covers the use of ab11338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 200 (l), 190 (s) kDa.
ICC/IF Use at an assay dependent dilution.

Target

  • FunctionBelongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton.
  • Involvement in diseaseNote=A chromosomal aberration involving MLLT4 is associated with acute leukemias. Translocation t(6;11)(q27;q23) with MLL/HRX. The result is a rogue activator protein.
  • Sequence similaritiesContains 1 dilute domain.
    Contains 1 FHA domain.
    Contains 1 PDZ (DHR) domain.
    Contains 2 Ras-associating domains.
  • DomainThe PDZ/DHR domain interacts with the C-terminus of nectin and the Pro-rich C-terminus domain interacts with F-actin.
  • Cellular localizationCell junction > adherens junction. Not found at cell-matrix AJs.
  • Information by UniProt
  • Database links
  • Alternative names
    • AF6 antibody
    • AFAD_HUMAN antibody
    • Afadin antibody
    • ALL1-fused gene from chromosome 6 protein antibody
    • Mllt4 antibody
    • Protein Af-6 antibody
    • Protein AF6 antibody
    see all

References for Anti-l + S Afadin antibody (ab11338)

This product has been referenced in:
  • Lachke SA  et al. The cell adhesion gene PVRL3 is associated with congenital ocular defects. Hum Genet : (2011). IHC-Fr ; Mouse . Read more (PubMed: 21769484) »
  • Sasaki AT  et al. Ubiquitination of K-Ras enhances activation and facilitates binding to select downstream effectors. Sci Signal 4:ra13 (2011). Read more (PubMed: 21386094) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab11338.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"