chicken NgCAM protein (ab24345 detects the C-terminus portion of the protein that is conserved with mammalian L1)
Our Abpromise guarantee covers the use of ab24345 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/300 - 1/1000.|
|IHC-FoFr||1/300 - 1/1000.|
|Flow Cyt||1/20. (paraformaldehyde or methanol fixed cells)
ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|WB||1/1000. Detects a band of approximately 200 kDa. Cleavage products observed at 60-80 kDa.|
This image is courtesy of Martin Grumet, Rutgers University, United States
ab24345 recognizes one or two polypeptides of L1 or Ng-CAM corresponding to the full length protein (~200kDa) as well as 60-80 kDa C-terminal cleavage products (as shown in the figure).
ab24345 (1/500) immunostaining L1CAM in 4% PFA-fixed rat cerebellum tissue. ML = molecular layer and FT = fiber tract of the cerebellum.