Anti-L1CAM antibody [UJ127] - BSA and Azide free (ab80832)
Key features and details
- Mouse monoclonal [UJ127] to L1CAM - BSA and Azide free
- Suitable for: ICC/IF, Flow Cyt, IHC-P, IHC-FoFr
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-L1CAM antibody [UJ127] - BSA and Azide free
See all L1CAM primary antibodies -
Description
Mouse monoclonal [UJ127] to L1CAM - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, Flow Cyt, IHC-P, IHC-FoFrmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human L1CAM. Homogenous suspension of 16 week Human fetal brain.
Database link: P32004 -
Positive control
- Neuroblastoma tissue.
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General notes
This product was changed from ascites to tissue culture supernatant on 17th August 2017. The following lots are from ascites and are still in stock on 17th August 2017 - GR3180729 and GR3174707. Lot numbers higher than GR3180729 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: 0.0268% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
UJ127 -
Myeloma
P3x63-Ag8.653 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab80832 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. PubMed: 6826247
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IHC-FoFr |
Use at an assay dependent concentration. PubMed: 24125017
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. PubMed: 6826247 |
IHC-FoFr
Use at an assay dependent concentration. PubMed: 24125017 |
Target
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Function
Cell adhesion molecule with an important role in the development of the nervous system. Involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Binds to axonin on neurons. -
Involvement in disease
Defects in L1CAM are the cause of hydrocephalus due to stenosis of the aqueduct of Sylvius (HSAS) [MIM:307000]. Hydrocephalus is a condition in which abnormal accumulation of cerebrospinal fluid in the brain causes increased intracranial pressure inside the skull. This is usually due to blockage of cerebrospinal fluid outflow in the brain ventricles or in the subarachnoid space at the base of the brain. In children is typically characterized by enlargement of the head, prominence of the forehead, brain atrophy, mental deterioration, and convulsions. In adults the syndrome includes incontinence, imbalance, and dementia. HSAS is characterized by mental retardation and enlarged brain ventricles.
Defects in L1CAM are the cause of mental retardation-aphasia-shuffling gait-adducted thumbs syndrome (MASA) [MIM:303350]; also known as corpus callosum hypoplasia, psychomotor retardation, adducted thumbs, spastic paraparesis, and hydrocephalus or CRASH syndrome. MASA is an X-linked recessive syndrome with a highly variable clinical spectrum. Main clinical features include spasticity and hyperreflexia of lower limbs, shuffling gait, mental retardation, aphasia and adducted thumbs. The features of spasticity have been referred to as complicated spastic paraplegia type 1 (SPG1). Some patients manifest corpus callosum hypoplasia and hydrocephalus. Inter- and intrafamilial variability is very wide, such that patients with hydrocephalus, MASA, SPG1, and agenesis of corpus callosum can be present within the same family.
Defects in L1CAM are the cause of spastic paraplegia X-linked type 1 (SPG1) [MIM:303350]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
Note=Defects in L1CAM may contribute to Hirschsprung disease by modifying the effects of Hirschsprung disease-associated genes to cause intestinal aganglionosis.
Defects in L1CAM are a cause of partial agenesis of the corpus callosum (ACCPX) [MIM:304100]. A syndrome characterized by partial corpus callosum agenesis, hypoplasia of inferior vermis and cerebellum, mental retardation, seizures and spasticity. Other features include microcephaly, unusual facies, and Hirschsprung disease in some patients. -
Sequence similarities
Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.
Contains 5 fibronectin type-III domains.
Contains 6 Ig-like C2-type (immunoglobulin-like) domains. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 3897 Human
- Omim: 308840 Human
- SwissProt: P32004 Human
- Unigene: 522818 Human
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Alternative names
- Antigen identified by monoclonal antibody R1 antibody
- CAML1 antibody
- CD171 antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab80832 has been referenced in 7 publications.
- Jiang C et al. Validation of α-Synuclein in L1CAM-Immunocaptured Exosomes as a Biomarker for the Stratification of Parkinsonian Syndromes. Mov Disord 36:2663-2669 (2021). PubMed: 33826157
- Jiang C et al. Serum neuronal exosomes predict and differentiate Parkinson's disease from atypical parkinsonism. J Neurol Neurosurg Psychiatry 91:720-729 (2020). PubMed: 32273329
- Shi M et al. Plasma exosomal a-synuclein is likely CNS-derived and increased in Parkinson's disease. Acta Neuropathol 128:639-650 (2014). PubMed: 24997849
- Yoo M et al. Analysis of human embryonic stem cells with regulatable expression of the cell adhesion molecule l1 in regeneration after spinal cord injury. J Neurotrauma 31:553-64 (2014). WB, IHC-FoFr ; Human . PubMed: 24125017
- Lee ES et al. A chimeric antibody to L1 cell adhesion molecule shows therapeutic effect in an intrahepatic cholangiocarcinoma model. Exp Mol Med 44:293-302 (2012). PubMed: 22248567
- Patel K et al. Monoclonal antibody UJ127.11 recognizes the human homologue of mouse L1 cell adhesion molecule. Biochem Soc Trans 18:274 (1990). Human . PubMed: 2379713
- Kemshead JT et al. Monoclonal antibody UJ 127:11 detects a 220,000-240,000 kdal. glycoprotein present on a sub-set of neuroectodermally derived cells. Int J Cancer 31:187-95 (1983). ICC/IF, IHC-P ; Human . PubMed: 6826247