Anti-L1CAM (phospho S1181) antibody (ab61009)

Overview

  • Product nameAnti-L1CAM (phospho S1181) antibody
    See all L1CAM primary antibodies
  • Description
    Rabbit polyclonal to L1CAM (phospho S1181)
  • SpecificityDetects endogenous levels of L1CAM only when phosphorylated at serine 1181.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human L1CAM. Amino acids sequence around the phosphosite serine 1181 (L-E-SP-D-N).
    Database link: P32004

  • Positive control
    • WB: extracts from K562 cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab61009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 172 kDa (predicted molecular weight: 140 kDa).

Target

  • FunctionCell adhesion molecule with an important role in the development of the nervous system. Involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Binds to axonin on neurons.
  • Involvement in diseaseDefects in L1CAM are the cause of hydrocephalus due to stenosis of the aqueduct of Sylvius (HSAS) [MIM:307000]. Hydrocephalus is a condition in which abnormal accumulation of cerebrospinal fluid in the brain causes increased intracranial pressure inside the skull. This is usually due to blockage of cerebrospinal fluid outflow in the brain ventricles or in the subarachnoid space at the base of the brain. In children is typically characterized by enlargement of the head, prominence of the forehead, brain atrophy, mental deterioration, and convulsions. In adults the syndrome includes incontinence, imbalance, and dementia. HSAS is characterized by mental retardation and enlarged brain ventricles.
    Defects in L1CAM are the cause of mental retardation-aphasia-shuffling gait-adducted thumbs syndrome (MASA) [MIM:303350]; also known as corpus callosum hypoplasia, psychomotor retardation, adducted thumbs, spastic paraparesis, and hydrocephalus or CRASH syndrome. MASA is an X-linked recessive syndrome with a highly variable clinical spectrum. Main clinical features include spasticity and hyperreflexia of lower limbs, shuffling gait, mental retardation, aphasia and adducted thumbs. The features of spasticity have been referred to as complicated spastic paraplegia type 1 (SPG1). Some patients manifest corpus callosum hypoplasia and hydrocephalus. Inter- and intrafamilial variability is very wide, such that patients with hydrocephalus, MASA, SPG1, and agenesis of corpus callosum can be present within the same family.
    Defects in L1CAM are the cause of spastic paraplegia X-linked type 1 (SPG1) [MIM:303350]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
    Note=Defects in L1CAM may contribute to Hirschsprung disease by modifying the effects of Hirschsprung disease-associated genes to cause intestinal aganglionosis.
    Defects in L1CAM are a cause of partial agenesis of the corpus callosum (ACCPX) [MIM:304100]. A syndrome characterized by partial corpus callosum agenesis, hypoplasia of inferior vermis and cerebellum, mental retardation, seizures and spasticity. Other features include microcephaly, unusual facies, and Hirschsprung disease in some patients.
  • Sequence similaritiesBelongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.
    Contains 5 fibronectin type-III domains.
    Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen identified by monoclonal antibody R1 antibody
    • CAML1 antibody
    • CD171 antibody
    • CD171 antigen antibody
    • HSAS antibody
    • HSAS1 antibody
    • Hyd antibody
    • L1 antibody
    • L1 cell adhesion molecule antibody
    • L1-NCAM antibody
    • L1cam antibody
    • L1CAM_HUMAN antibody
    • MASA antibody
    • MIC5 antibody
    • N CAML1 antibody
    • N-CAM-L1 antibody
    • NCAM-L1 antibody
    • NCAML1 antibody
    • Nerve-growth factor-inducible large external glycoprotein antibody
    • Neural cell adhesion molecule L1 antibody
    • NILE antibody
    • OTTHUMP00000025992 antibody
    • S10 antibody
    • SPG1 antibody
    see all

Anti-L1CAM (phospho S1181) antibody images

  • All lanes : Anti-L1CAM (phospho S1181) antibody (ab61009) at 1/500 dilution

    Lane 1 : extracts from K562 cell
    Lane 2 : extracts from K562 cell with immunizing phosphopeptide


    Predicted band size : 140 kDa
    Observed band size : 172 kDa (why is the actual band size different from the predicted?)

References for Anti-L1CAM (phospho S1181) antibody (ab61009)

ab61009 has not yet been referenced specifically in any publications.

Product Wall

Thanks for your email.

While processing the refund request, I have found that we have already provided a free of charge replacement antibody, ab29306, to replace the vial of ab61009 that was not working. This replacement was shippedon Abcam o...

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Thanks so much for the follow up email.

I agree that the vials of ab61009 you received do not seem to be specific for the phosphorylated form of L1CAM. Again Iam very sorry for the trouble here. I am happy to offer a replacement of any primar...

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Thank you for your call today and for letting us know about the trouble with ab61009.

As we discussed, I'm sending a free of charge vial of the K562 cell lysate on the order ***, which should arrive tomorrow.

Please keep me updated ...

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