Overview

  • Product name
  • Description
    Mouse polyclonal to L3MBTL1
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to amino acids 1-772 of Human L3MBTL1 Isoform 1. (AAH39820.1).

  • Positive control
    • Transfected 293T cell lysate; HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 7.20
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab167285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 84.92 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Polycomb group (PcG) protein that specifically recognizes and binds mono- and dimethyllysine residues on target proteins, therey acting as a 'reader' of a network of post-translational modifications. PcG proteins maintain the transcriptionally repressive state of genes: acts as a chromatin compaction factor by recognizing and binding mono- and dimethylated histone H1b/HIST1H1E at 'Lys-26' (H1bK26me1 and H1bK26me2) and histone H4 at 'Lys-20' (H4K20me1 and H4K20me2), leading to condense chromatin and repress transcription. Recognizes and binds p53/TP53 monomethylated at 'Lys-382', leading to repress p53/TP53-target genes. Also recognizes and binds RB1/RB monomethylated at 'Lys-860'. Participates in the ETV6-mediated repression. Probably plays a role in cell proliferation. Overexpression induces multinucleated cells, suggesting that it is required to accomplish normal mitosis.
  • Tissue specificity
    Widely expressed. Expression is reduced in colorectal cancer cell line SW480 and promyelocytic leukemia cell line HL-60.
  • Sequence similarities
    Contains 1 C2HC-type zinc finger.
    Contains 3 MBT repeats.
    Contains 1 SAM (sterile alpha motif) domain.
  • Developmental stage
    In interphase cells, it is scattered throughout the nucleoplasm. In mitotic cells, it strongly associates with condensed chromosomes from the prophase to telophase.
  • Domain
    The MBT repeat 2 specifically recognizes and binds monomethylated and dimethylated proteins. In contrast, it does not bind trimethylated proteins. The MBT repeat 1 does not bind methylated peptides but inserts a proline ring in a Pro-Ser-Ser/Thr sequence context.
  • Cellular localization
    Nucleus. Excluded from the nucleolus. Does not colocalizes with the PcG protein BMI1, suggesting that these two proteins do not belong to the same complex.
  • Information by UniProt
  • Database links
  • Alternative names
    • dJ138B7.3 antibody
    • H-L(3)MBT antibody
    • H-l(3)mbt protein antibody
    • KIAA0681 antibody
    • L(3)mbt protein homolog antibody
    • L(3)mbt-like antibody
    • L3MBT antibody
    • L3MBTL antibody
    • L3MBTL1 antibody
    • Lethal (3) malignant brain tumor l(3 antibody
    • Lethal(3)malignant brain tumor-like protein 1 antibody
    • LMBL1_HUMAN antibody
    • RGD1307316 antibody
    • ZC2HC3 antibody
    see all

Images

  • All lanes : Anti-L3MBTL1 antibody (ab167285) at 1 µg/ml

    Lane 1 : Transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Secondary
    Goat Anti-mouse HRP at 1/2500 dilution

References

ab167285 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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