• Product nameAnti-L3MBTL1 antibody
    See all L3MBTL1 primary antibodies
  • Description
    Rabbit polyclonal to L3MBTL1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 365 and 723 of L3MBTL.

  • Positive control
    • 293T, A431, H1299, HeLaS3, HepG2, MOLT4 or Raji cell lysate; ICC/IF: HeLa cells.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab97304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 84 kDa.
ICC/IF 1/100 - 1/200.


  • FunctionPolycomb group (PcG) protein that specifically recognizes and binds mono- and dimethyllysine residues on target proteins, therey acting as a 'reader' of a network of post-translational modifications. PcG proteins maintain the transcriptionally repressive state of genes: acts as a chromatin compaction factor by recognizing and binding mono- and dimethylated histone H1b/HIST1H1E at 'Lys-26' (H1bK26me1 and H1bK26me2) and histone H4 at 'Lys-20' (H4K20me1 and H4K20me2), leading to condense chromatin and repress transcription. Recognizes and binds p53/TP53 monomethylated at 'Lys-382', leading to repress p53/TP53-target genes. Also recognizes and binds RB1/RB monomethylated at 'Lys-860'. Participates in the ETV6-mediated repression. Probably plays a role in cell proliferation. Overexpression induces multinucleated cells, suggesting that it is required to accomplish normal mitosis.
  • Tissue specificityWidely expressed. Expression is reduced in colorectal cancer cell line SW480 and promyelocytic leukemia cell line HL-60.
  • Sequence similaritiesContains 1 C2HC-type zinc finger.
    Contains 3 MBT repeats.
    Contains 1 SAM (sterile alpha motif) domain.
  • Developmental stageIn interphase cells, it is scattered throughout the nucleoplasm. In mitotic cells, it strongly associates with condensed chromosomes from the prophase to telophase.
  • DomainThe MBT repeat 2 specifically recognizes and binds monomethylated and dimethylated proteins. In contrast, it does not bind trimethylated proteins. The MBT repeat 1 does not bind methylated peptides but inserts a proline ring in a Pro-Ser-Ser/Thr sequence context.
  • Cellular localizationNucleus. Excluded from the nucleolus. Does not colocalizes with the PcG protein BMI1, suggesting that these two proteins do not belong to the same complex.
  • Information by UniProt
  • Database links
  • Alternative names
    • dJ138B7.3 antibody
    • H-L(3)MBT antibody
    • H-l(3)mbt protein antibody
    • KIAA0681 antibody
    • L(3)mbt protein homolog antibody
    • L(3)mbt-like antibody
    • L3MBT antibody
    • L3MBTL antibody
    • L3MBTL1 antibody
    • Lethal (3) malignant brain tumor l(3 antibody
    • Lethal(3)malignant brain tumor-like protein 1 antibody
    • LMBL1_HUMAN antibody
    • RGD1307316 antibody
    • ZC2HC3 antibody
    see all

Anti-L3MBTL1 antibody images

  • Anti-L3MBTL1 antibody (ab97304) at 1/1000 dilution + Hela S3 whole cell lysate at 30 µg

    Predicted band size : 84 kDa
  • Images show immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using 1/200 ab97304 alone (1) or merged with DNA probe (2) .

References for Anti-L3MBTL1 antibody (ab97304)

ab97304 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: RT°C
Sample Mouse Cell (Primary cells)
Specification Primary cells
Permeabilization Yes - Triton X-100
Fixative Paraformaldehyde

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Submitted Jun 07 2013