Overview

  • Product nameAnti-L3MBTL1 antibody - ChIP Grade
    See all L3MBTL1 primary antibodies
  • Description
    Rabbit polyclonal to L3MBTL1 - ChIP Grade
  • Tested applicationsSuitable for: WB, IP, ChIPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: all other Vertebrates
  • Immunogen

    His-tagged fusion protein corresponding to amino acids 1-215 of human L3MBTL1

  • Positive control
    • Nuclear extract of HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab51880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Detects a band of approximately 100 kDa (predicted molecular weight: 86 kDa). Slight reactivity of a 70 kDa protein is also observed.
IP Use at an assay dependent dilution.
ChIP Use at an assay dependent dilution.

Target

  • FunctionPolycomb group (PcG) protein that specifically recognizes and binds mono- and dimethyllysine residues on target proteins, therey acting as a 'reader' of a network of post-translational modifications. PcG proteins maintain the transcriptionally repressive state of genes: acts as a chromatin compaction factor by recognizing and binding mono- and dimethylated histone H1b/HIST1H1E at 'Lys-26' (H1bK26me1 and H1bK26me2) and histone H4 at 'Lys-20' (H4K20me1 and H4K20me2), leading to condense chromatin and repress transcription. Recognizes and binds p53/TP53 monomethylated at 'Lys-382', leading to repress p53/TP53-target genes. Also recognizes and binds RB1/RB monomethylated at 'Lys-860'. Participates in the ETV6-mediated repression. Probably plays a role in cell proliferation. Overexpression induces multinucleated cells, suggesting that it is required to accomplish normal mitosis.
  • Tissue specificityWidely expressed. Expression is reduced in colorectal cancer cell line SW480 and promyelocytic leukemia cell line HL-60.
  • Sequence similaritiesContains 1 C2HC-type zinc finger.
    Contains 3 MBT repeats.
    Contains 1 SAM (sterile alpha motif) domain.
  • Developmental stageIn interphase cells, it is scattered throughout the nucleoplasm. In mitotic cells, it strongly associates with condensed chromosomes from the prophase to telophase.
  • DomainThe MBT repeat 2 specifically recognizes and binds monomethylated and dimethylated proteins. In contrast, it does not bind trimethylated proteins. The MBT repeat 1 does not bind methylated peptides but inserts a proline ring in a Pro-Ser-Ser/Thr sequence context.
  • Cellular localizationNucleus. Excluded from the nucleolus. Does not colocalizes with the PcG protein BMI1, suggesting that these two proteins do not belong to the same complex.
  • Information by UniProt
  • Database links
  • Alternative names
    • dJ138B7.3 antibody
    • H-L(3)MBT antibody
    • H-l(3)mbt protein antibody
    • KIAA0681 antibody
    • L(3)mbt protein homolog antibody
    • L(3)mbt-like antibody
    • L3MBT antibody
    • L3MBTL antibody
    • L3MBTL1 antibody
    • Lethal (3) malignant brain tumor l(3 antibody
    • Lethal(3)malignant brain tumor-like protein 1 antibody
    • LMBL1_HUMAN antibody
    • RGD1307316 antibody
    • ZC2HC3 antibody
    see all

Anti-L3MBTL1 antibody - ChIP Grade images

  • Anti-L3MBTL1 antibody - ChIP Grade (ab51880) at 1/2000 dilution + Nuclear extract of HeLa cells at 20 µg

    Predicted band size : 86 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 70 kDa (possible cross reactivity).

References for Anti-L3MBTL1 antibody - ChIP Grade (ab51880)

This product has been referenced in:
  • Liu W  et al. PHF8 mediates histone H4 lysine 20 demethylation events involved in cell cycle progression. Nature 466:508-12 (2010). WB ; Human . Read more (PubMed: 20622854) »
  • Saddic LA  et al. Methylation of the retinoblastoma tumor suppressor by SMYD2. J Biol Chem 285:37733-40 (2010). WB ; Human . Read more (PubMed: 20870719) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (fibroblast)
Specification fibroblast
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jun 17 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"