Overview

  • Product name
  • Description
    Rabbit polyclonal to L3MBTL2
  • Tested applications
    Suitable for: IHC-P, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 655 and 705 of Human L3MBTL2 (NP_113676.2).

  • Positive control
    • HeLa whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab101272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Putative Polycomb group (PcG) protein. PcG proteins maintain the transcriptionally repressive state of genes, probably via a modification of chromatin, rendering it heritably changed in its expressibility. Its association with a chromatin-remodeling complex suggests that it may contribute to prevent expression of genes that trigger the cell into mitosis. Binds to monomethylated and dimethylated 'Lys-20' on histone H4. Binds histone H3 peptides that are monomethylated or dimethylated on 'Lys-4', 'Lys-9' or 'Lys-27'.
    • Sequence similarities
      Contains 1 FCS-type zinc finger.
      Contains 4 MBT repeats.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • 3bt like 2 protein antibody
      • 3bt like protein antibody
      • DKFZP761I141 antibody
      • H-l(3)mbt-like protein 2 antibody
      • H-l(3)mbt-like protein antibody
      • Hl antibody
      • Hl(3)mbt like protein antibody
      • Hl(3)mbtl antibody
      • L antibody
      • l(3)mbt like 2 (Drosophila) antibody
      • l(3)mbt like 2 antibody
      • L(3)mbt-like 2 protein antibody
      • L(3)mbt-like protein 2 antibody
      • L3MBTL2 antibody
      • Lethal antibody
      • Lethal(3)malignant brain tumor-like 2 protein antibody
      • Lethal(3)malignant brain tumor-like protein 2 antibody
      • LMBL2_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling L3MBTL2 with ab101272 at 1/1000 (1µg/ml).
    • Detection of L3MBTL2 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab101272 at 3 µg/mg lysate for IP (Lane 1). An anti-L3MBTL2 antibody which recognizes an upstream epitope was used in Lane 2, and also for the subsequent WB detection at 1 µg/ml. Lane 3 represents control IgG. Detection: Chemiluminescence with an exposure time of 30 seconds.

    References

    ab101272 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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