Anti-L3MBTL3 antibody (ab68117)
Key features and details
- Mouse polyclonal to L3MBTL3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-L3MBTL3 antibody
See all L3MBTL3 primary antibodies -
Description
Mouse polyclonal to L3MBTL3 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human L3MBTL3. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab68117 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 88 kDa).
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 88 kDa). |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Putative Polycomb group (PcG) protein. PcG proteins maintain the transcriptionally repressive state of genes, probably via a modification of chromatin, rendering it heritably changed in its expressibility. Required for normal maturation of myeloid progenitor cells. -
Sequence similarities
Contains 3 MBT repeats.
Contains 1 SAM (sterile alpha motif) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 84456 Human
- SwissProt: Q96JM7 Human
- Unigene: 658051 Human
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Alternative names
- L3MBTL3 antibody
- H-l(3)mbt-like protein 3 antibody
- Hl(3)mbt like protein antibody
see all
Images
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All lanes : Anti-L3MBTL3 antibody (ab68117) at 1/500 dilution
Lane 1 : L3MBTL3 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary
antibody at 1/2500 dilution
Predicted band size: 88 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted? -
Immunofluorescence analysis of HeLa cells using purified ab68117 at 10 µg/ml followed by fluorescein conjugated secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilised with 0.1% Triton X-100.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab68117 has not yet been referenced specifically in any publications.